Functionalized microfluidic device and method

1. A microfluidic platform, comprising:

a base having:

an outer surface;

a well formed in the outer surface of the base for receiving a fluid therein, the well having an open end communicating with the outer surface of the base and being configured to allow the fluid to flow therepast, and a closed end being configured to prevent the fluid from flowing therepast; and

a recess in the outer surface of the base at a location spaced from the well, the recess having an open end communicating with the outer surface of the base and being configured to allow the fluid to flow therepast, and a closed end being configured to prevent the fluid from flowing therepast;

an absorbent received in the recess in the base;

a lid having an interior, an outer surface and a channel extending through the interior and being spaced from the outer surface of the lid;

an input port projecting from the outer surface of the lid, terminating at an end surface and having a passageway therethrough, the passageway of the input port having a first end communicating an input of the channel and a second end communicating with the end surface of the input port; and

an output port projecting from the outer surface of the lid and terminating at an end surface, the output port having:

a passageway extending therethrough, the passageway of the output port having a first end communicating with an output of the channel and a second end communicating with the end surface of the output port; and

an output outer surface of the output port having a slot therein extending between the outer surface of the lid and the end surface of the output port, the slot communicating with the passageway of the output port;

wherein:

the lid selectively moveable between a first position wherein the input port projecting from the lid is disengaged from the base and a second position wherein:

the outer surface of the lid and the outer surface of the base are in spaced relation wherein the slot in the output outer surface of the output port communicates with an environment external of the lid and the base;

the fluid is allowed to pass through the slot in the output outer surface of the output port extending from the lid; and

the input of the channel communicates with the fluid in the well through the passageway of the input port and the output of the channel communicates with the absorbent in the recess through the passageway of the output port;

wherein:

with the lid in the second position:

the second end of the input port communicates with the fluid in the well of the base such that the fluid in the well is drawn into the passageway of the input port by capillary action;

the absorbent drives fluid flow from the well into the passageway of the input port through the channel in the lid and into the passageway of the output port; and

the slot in the output outer surface of the output port allows for the environment to be received in the slot while allowing for a fluid connection between the absorbent and the fluid in the well.

2. The microfluidic platform of claim 1 further comprising a removable membrane connected to the outer surface of the base and extending over the well for retaining the fluid therein.

3. The microfluidic platform of claim 1 wherein the input port defines a post, the post receivable in the well with the lid in the second position.

4. A microfluidic platform, comprising:

a base having:

an outer surface;

a plurality of wells formed in the outer surface of the base for receiving fluid therein, each well of the plurality of wells having an open end in fluid communication with the outer surface of the base and being configured to allow the fluid to flow therepast, and a closed end being configured to prevent fluid from flowing therepast; and

at least one recess in the outer surface of the base at a location spaced from the plurality of wells, each of the at least one recess having an open end communicating with the outer surface of the base and being configured to allow the fluid to flow therepast, and a closed end being configured to prevent the fluid from flowing therepast;

an absorbent received in the at least one recess in the base; and

a lid including an interior, an outer surface and a plurality of channels extending through the interior being spaced from the outer surface of the lid and having corresponding inputs and outputs;

input ports projecting from the outer surface of the lid and terminating at corresponding end surfaces, each input port including a passageway therethrough having a first end communicating an input of a corresponding channel of the plurality of channels and a second end communicating with the corresponding end surface; and

output ports projecting from the outer surface of the lid and terminating at corresponding end surfaces, each output port having:

a passageway extending therethrough, the passageway of each output port having a first end communicating with an output of a corresponding channel of the plurality of channels and a second end communicating with the corresponding end surface; and

an output outer surface of a corresponding output port having a slot therein extending between the outer surface of the lid and the end surface of the corresponding output port, the slot communicating with the passageway through the corresponding output port;

the lid is selectively moveable between a first position wherein the input ports of the lid are disengaged from the base and a second position wherein:

the outer surface of the lid and the outer surface of the base are in spaced relation wherein the slots in the output outer surfaces of the corresponding output ports of the lid communicate with an environment external of the base and the lid;

the fluid is allowed to pass through the slots in the output outer surfaces of the corresponding output ports of the lid; and

the input of each channel of the plurality of channels communicates with a corresponding well of the plurality of wells in the base through the passageway of a corresponding input port and the output of each channel of the plurality of channels communicates with the absorbent in the at least one recess through the passageway of the corresponding output port;

the fluid in each well of the plurality of wells is drawn into a corresponding channel of the plurality of channels through the passageway of the corresponding input port, the input of the corresponding channel of the plurality of channels through the lid, and the passageway of the corresponding output port;

the absorbent drives fluid flow through the plurality of channels in the lid; and

the slots in the output outer surfaces of the corresponding output ports allow for the environment to be received in the slots while allowing for fluid connections between the absorbent and the fluid in each well.

5. The microfluidic platform of claim 4 further comprising a removable membrane connected to the outer surface of the base and extending over the plurality of wells for retaining the fluid therein.

6. The microfluidic platform of claim 4 wherein each input port defines a post, each post receivable in the corresponding well of the plurality of wells with the lid in the second position.