Method for monitoring minimal residual hairy cell leukemia
The present invention relates to hairy cell leukemia biomarkers and methods of utilizing these biomarkers to diagnose and/or treat hairy cell leukemia.
1. A method of monitoring minimal residual disease in a subject who has been treated for hairy cell leukemia with an anti-proliferative agent, the method comprising:
obtaining a first urine sample from the subject, and
detecting the BRAF V600E mutation, if present, in said sample,
wherein said detecting comprises amplifying, by the polymerase chain reaction (PCR), a nucleic acid encoding said BRAF V600E mutation in said sample,
wherein the detection of the BRAF V600E mutation indicates the presence of residual hairy cell leukemia in the subject and
wherein the treatment with the anti-proliferative agent ended and no signs or symptoms of hairy cell leukemia was evident when the first urine sample was taken.
2. The method of claim 1 , further comprising comparing the amount of said BRAF V600E mutation in said first urine sample with the amount of said BRAF V600E mutation determined in a urine sample from a subject not suffering from hairy cell leukemia or symptoms thereof.
3. The method of claim 1 , further comprising sequencing said BRAF V600E mutation in the nucleic acids amplified by PCR.
4. The method of claim 1 , further comprising administering a therapeutically effective amount of an anti-proliferative agent to said subject if the BRAF V600E mutation is in the sample.
5. The method of claim 4 , wherein said anti-proliferative agent is a purine analog, and interferon, rituximab or bendamustine.
6. The method of claim 5 , wherein said purine analog is pentostatin, cladaribine, azathioprine, mercaptopurine, thioguanine or fludarabine.
7. The method of claim 4 , further comprising administering a therapeutically effective amount of a BRAF inhibitor.
8. The method of claim 7 , wherein said BRAF inhibitor is PLX-4032 (Vemurafenib), GSK 2118436 (Dabrafenib), PLX-4720, SB590885, XL-281, RAF-265, GDC-0897, or Sorafenib.
9. The method of claim 4 , further comprising administering a therapeutically effective amount of a MEK or ERK inhibitor.
10. The method of claim 9 , wherein said MEK or ERK inhibitor is Any-142886/AZD-6244, SCIO-469, GW681323, U0126, XL-518, CI-1040, PD035901 or GSK1120212.
11. The method of claim 1 , further comprising recommending the administration of a therapeutically effective amount of an anti-proliferative agent to said subject if the BRAF V600E mutation is present in the sample.