Methods for treating atrial or ventricular arrhythmias by administering a G-protein alpha inhibitor
The present invention provides compositions and methods for treating heart conditions. In particular, the present invention provides compositions and methods that block G protein coupled receptor mediated signaling for treating atrial fibrillation.
1. A method for treating a subject having atrial or ventricular arrhythmias, comprising administering said subject an effective amount of a G-protein inhibitor, wherein said G-protein inhibitor comprises a Gαo1 inhibitor, and wherein said administering is under conditions such that symptoms of said atrial or ventricular arrhythmias are reduced or eliminated.
2. The method of claim 1 , wherein said arrhythmia comprises atrial fibrillation.
3. The method of claim 1 , wherein exposing said subject to said G-protein inhibitor disrupts one or more autonomic pathways.
4. The method of claim 3 , wherein said autonomic pathways comprise sympathetic or parasympathetic pathways.
5. The method of claim 1 , wherein said exposing comprises local administration.
6. The method of claim 5 , further comprising electroporation of the site of said local administration.
7. The method of claim 1 , wherein said G-protein inhibitor comprises a G-protein inhibitory peptide.
8. The method of claim 1 , further comprising administering a Gαi2 inhibitor to said subject.
9. The method of claim 7 , wherein said G-protein inhibitory peptide blocks receptor/G protein interaction.
10. The method of claim 1 , wherein said G-protein inhibitor comprises a nucleic acid molecule encoding a G-protein inhibitory peptide.
11. The method of claim 1 , wherein said exposing comprises topical administration.
12. The method of claim 1 , wherein said subject is undergoing open-heart surgery.
13. The method of claim 1 , wherein said subject has a clinical history of a vagal or adrenergic system role associated with said atrial arrhythmias.
14. The method of claim 1 , wherein said subject has a paroxysmal or chronic history of atrial fibrillation.
15. The method of claim 1 , wherein said exposing comprises treatment of autonomic pathways: a) within the left or right atrium of the heart; b) adjacent to the atria, or c) at one or more sites distant from the atria but that innervate the atria.
16. The method of claim 1 , wherein said exposing comprises treatment of autonomic pathways in ventricular arrhythmias.
17. A method for treating a subject having atrial fibrillation comprising locally administering a G-protein inhibitor to the heart of said subject, and electroporating the site of administration, wherein said G-protein inhibitor comprises a Gαo inhibitor.
18. The method of claim 17 , wherein said G-protein inhibitor is applied topically using a catheter or injection apparatus.
19. The method of claim 17 , wherein said G-protein inhibitor comprises an inhibitory peptide or nucleic acid.
20. The method of claim 17 , wherein said administering is under conditions such that symptoms of said atrial or ventricular arrhythmias are reduced.