Target and method for inhibition of bacterial RNA polymerase
View Patent ↗Target and method for inhibition of bacterial RNA polymerase disclosed are targets and methods for specific binding and inhibition of RNAP from bacterial species.
1. An isolated analog of bacteriocidal-peptide microcin J25 (MccJ25) that (1) has an amino acid sequence that differs from that of MccJ25 in terms of at least one amino acid substitution, insertion, or deletion; and (2) that binds a bacterial RNAP and inhibits an activity of bacterial RNAP with a potency at least equal to that of MccJ25 wherein the analog is selected from the group consisting of [Lys 5 ]MccJ25, [Lys 13 ]MccJ25, [Lys 15 ]MccJ25, and [Lys 17 ]MccJ25.
2. The isolated analog according to claim 1 that also contains a detectable group.
3. The isolated analog according to claim 2 wherein the detectable group is selected from the group consisting of a chromophore, fluorophore and Cy3.
4. An isolated analog of bacteriocidal-peptide microcin J 25 (MccJ25) that (1) has an amino acid sequence that differs from that of MccJ25 in terms of at least one amino acid substitution, insertion, or deletion; and (2) that binds a bacterial RNAP and inhibits an activity of bacterial RNAP with a potency at least equal to that of MccJ 25 wherein the analog is selected from the group consisting of [X-Lys 5 ]MccJ25, [X-Lys 13 ]MccJ25, [X-Lys 15 ]MccJ25, and [X-Lys 17 ]MccJ25, wherein X is a detectable group.
5. The isolated analog according to claim 4 wherein the detectable group is selected from the group consisting of a chromophore, fluorophore and Cy3.