Polypeptides having cellobiohydrolase 1 activity and polynucleotides encoding same
The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
1. An isolated polypeptide having cellobiohydrolase I activity, which has at least 80% identity with amino acids 1 to 457 of SEQ ID NO: 8.
2. The polypeptide of claim 1 , which has at least 85% identity with amino acids 1 to 457 of SEQ ID NO: 8.
3. The polypeptide of claim 1 , which has at least 90% identity with amino acids 1 to 457 of SEQ ID NO: 8.
4. The polypeptide of claim 1 , which has at least 95% identity with amino acids 1 to 457 of SEQ ID NO: 8.
5. The polypeptide of claim 1 , which has at least 97% identity with amino acids 1 to 457 of SEQ ID NO: 8.
6. The polypeptide of claim 1 , which has at least 98% identity with amino acids 1 to 457 of SEQ ID NO: 8.
7. The polypeptide of claim 1 , which has at least 99% identity with amino acids 1 to 457 of SEQ ID NO: 8.
8. The polypeptide of claim 1 , comprising the sequence of amino acids 1 to 457 of SEQ ID NO: 8.
9. The polypeptide of claim 1 , which is a fragment of the sequence of amino acids 1 to 457 of SEQ ID NO: 8.
10. The polypeptide of claim 1 , which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of:
(i) the complementary strand of nucleotides 1 to 1371 of SEQ ID NO: 7,
(ii) the complementary strand of nucleotides 1 to 500 of SEQ ID NO: 7, and
(iii) the complementary strand of nucleotides 1 to 200 of SEQ ID NO: 7;
wherein the high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 1.0% SDS, 5×Denhardt's solution, 100 μg/ml sheared and denatured salmon sperm DNA, following standard Southern blotting procedures, followed by washing three times each for 15 minutes using 0.1×SSC, 0.1% SDS at 60° C.
11. A detergent composition comprising a surfactant and the polypeptide of claim 1 .
12. A method for producing ethanol from biomass, comprising
(a) contacting the biomass with the polypeptide of claim 1 , an endo-1,4-beta-glucanase, and a beta-D-glucosidase to produce sugar; and
(b) fermenting the sugar to produce ethanol.