Methods and materials for increasing potency of cells
View Patent ↗Disclosed herein are methods and materials for producing a more developmentally potent cell from a less developmentally potent cell. Specifically exemplified herein are methods that comprise introducing an expressible dedifferentiating polynucleotide sequence into a less developmentally potent cell, wherein the transfected less developmentally potent cell becomes a more developmentally potent cell capable of differentiating to a less developmentally potent cell of its lineage of origin or a different lineage.
1. A method of transforming an adult mesenchymal stem cell into a pluripotent cell, said method comprising introducing into said adult mesenchymal stem cell an oct4 gene and a sox 2 gene and culturing said adult mesenchymal stem cell under conditions to produce expression of said oct4 and sox 2 genes, wherein said expression results in said adult mesenchymal stem cell becoming a pluripotent cell.