Method for producing differentiated cells
The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.
1. A method of producing mature megakaryocytic cells from megakaryocytic progenitor cells without polyploidization comprising:
differentiating megakaryocytic progenitor cells without polyploidization by (i) inducing expression of an MYC family oncogene and polycomb complex protein-1 (BMI1)
in the megakaryocytic progenitor cells without polyploidization; and (ii) culturing the cells.
2. The method according to claim 1 , wherein the culturing comprises:
culturing the cells by suppressing the expression of the MYC family oncogene and the BMI1; or
culturing the cells after the MYC family oncogene and the BMI1 have been removed from the cells.
3. The method according to claim 1 further comprising cryopreserving the grown cells after the differentiating step, wherein the culturing is conducted after the cells are thawed.
4. A method of producing a platelet product comprising:
producing mature megakaryocytic cells by the method according to any one of claims 1 - 3 ;
recovering from the culture of the megakaryocytic cells a fraction of a culture solution in which platelets released from megakaryocytic cells are abundant; and
removing blood cell components other than platelets from the fraction.
5. A method of producing a blood product comprising:
producing a platelet product by the method according to claim 4 ; and
mixing the platelet product with other components of a blood product.
6. The method according to claim 1 , wherein the MYC family oncogene is c-MYC oncogene.
7. The method according to claim 4 , wherein the MYC family oncogene is c-MYC oncogene.
8. The method according to claim 5 , wherein the MYC family oncogene is c-MYC oncogene.
9. The method according to claim 2 further comprising cryopreserving the grown cells after the differentiating step, wherein the culturing is conducted after the cells are thawed.
10. A method of producing a platelet product comprising:
producing mature megakaryocytic cells by the method according to claim 9 ;
recovering from the culture of the megakaryocytic cells a fraction of a culture solution in which platelets released from megakaryocytic cells are abundant; and
removing blood cell components other than platelets from the fraction.
11. A method of producing a blood product comprising:
producing a platelet product by the method according to claim 10 ; and
mixing the platelet product with other components of a blood product.