Agents from cells
A composition includes an isolated cell; at least one particle within said cell; and at least one active agent associated with the particle, wherein the active agent is capable of being released from the cell. A method includes administration of such a cell to a subject.
1. A composition comprising:
a) an isolated mammalian cell;
b) at least one particle comprising a polymer and having a size of about 1 μm to 2 μm within the cell; and
c) at least one active agent associated with the particle;
wherein the active agent is capable of being released from inside the cell independently of the particle.
2. The composition of claim 1 , wherein the particle is formulated to release the agent into the cell in a controlled or predictable manner.
3. The composition of claim 1 , wherein the particle is formulated to release the agent to the extracellular environment.
4. The composition of claim 1 , wherein the agent is a therapeutic agent, diagnostic agent, imaging agent, chemoattractant, homing agent, immunomodulator, cytotoxic agent, or an angiogenesis inhibitor.
5. The composition of claim 1 , wherein the agent is covalently attached to the particle.
6. The composition of claim 1 , wherein the agent is noncovalently entrapped in the particle.
7. The composition of claim 1 , wherein the cell is a stem cell or a progenitor cell.
8. The composition of claim 1 , further comprising at least one extracellular particle not on or within the cell.
9. The composition of claim 8 , wherein the at least one extracellular particle is associated with an encapsulating material.
10. The composition of claim 1 , wherein the composition is cryopreserved.
11. The composition of claim 7 , wherein the stem cell is a mesenchymal stem cell.
12. The composition of claim 1 , wherein the polymer is poly (lactide-co-glycolide).
13. The composition of claim 1 , wherein the particle is formulated to release the agent from inside the cell over a period of at least 10 days.