Composition for inducing tissue regeneration by activating platelet-rich plasma (PRP)
The present invention relates to a composition for cartilaginous tissue repair and to a production method therefor. The present invention comprises the steps of: (a) dissolving freeze-dried fibrinogen in an aprotinin solution; (b) dissolving freeze-dried thrombin in a stabilizing solution; (c) mixing an enriched collagen solution with thrombin and the stabilizing solution; and installing the fibrinogen solution (a) to one side of a dual kit and the solution (c) containing the collagen to the other side, and then mixing and injecting into damaged cartilaginous tissue. In the present invention, which is constituted as described above, biomaterials such as collagen and fibrin are mixed so as to allow damaged cartilaginous tissue to be repaired to a state allowing transplantation onto the tissue, and efficient regeneration is induced, thereby making it possible to reduce surgery-related stress on people and animals while inducing relatively rapid and efficient cartilage repair and regeneration.
1. A method of manufacturing a composition for inducing tissue regeneration by activating platelet rich plasma (PRP), the method comprising the steps of:
separating the PRP from whole blood, wherein the separating step further comprises the steps of:
collecting 10 ml of the whole blood from an animal or patient into a vacuum test tube containing 3.2% sodium citrate, and primarily centrifuging the collected whole blood at 1,750-1,900 g for 3 to 5 minutes;
collecting a supernatant liquid comprising a plasma layer with a buffy coat obtained from said centrifugation;
transferring the collected supernatant liquid to a new vacuum test tube by a blunt needle, and secondarily centrifuging the collected supernatant liquid at 4,500-5,000 g for 4 to 6 minutes; and
collecting the PRP concentrated in a bottom layer by another blunt needle;
mixing 1 mL of the PRP collected from the separating step with a calcium chloride solution with a concentration of 0.30-0.55 mg/mL by a three-way connector; and
mixing a mixture of the PRP and the calcium chloride solution with type I collagen, wherein the mixing step of mixing the mixture of the PRP and the calcium chloride solution with the type I collagen further comprises the steps of:
leaving the type I collagen at a room temperature for 15 to 30 minutes before mixing; and
mixing the mixture of the PRP and the calcium chloride solution with the type I collagen with a concentration of 20-50 mg/mL, in an opaque phase, four times by another three-way connector.
2. The method of claim 1 , further comprising the step of:
injecting the mixture of the PRP, the calcium chloride solution and the type I collagen, charged in a syringe, into all regions in need of the tissue regeneration.