Porous thin films
View Patent ↗Compositions of porous thin films and methods of making are provided. The methods involve self-assembly of a cyclic peptide in the presence of a block copolymer.
1. A method of making a porous thin film, the method comprising:
contacting a substrate with a mixture comprising
a plurality of cyclic peptide conjugates each comprising a cyclic peptide and a conjugate polymer covalently linked to the cyclic peptide, and
a plurality of block copolymers each block copolymer comprising a first block able to bind to a conjugate polymer of the plurality of cyclic peptide conjugates and a second block that does not substantially bind to the conjugate polymer; and
annealing the mixture, such that the plurality of cyclic peptide conjugates self-assemble vertically in horizontal planes to form a plurality of cyclic peptide nanotubes perpendicular to the substrate and extending from a top surface to a bottom surface of the porous thin film.
2. The method of claim 1 , wherein the cyclic peptide comprises alternating D and L amino acids.
3. The method of claim 1 , wherein the cyclic peptide comprises alanine and lysine.
4. The method of claim 3 , wherein the cyclic peptide has the formula:
[D-Ala-L-Lys] x
wherein x is an integer from 3 to 10.
5. The method of claim 4 , wherein the cyclic peptide is selected from the group consisting of [D-Ala-L-Lys] 4 and [D-Ala-L-Lys] 5 .
6. The method of claim 4 , wherein the cyclic peptide is [D-Ala-L-Lys] 4 .
7. The method of claim 1 , wherein the conjugate polymer is a hydrophilic polymer.
8. The method of claim 1 , wherein the conjugate polymer is selected from the group consisting of polyethylene oxide (PEO), polymethylmethacrylate (PMMA) and polystyrene (PS).
9. The method of claim 8 , wherein the conjugate polymer is polyethylene oxide (PEO).
10. The method of claim 1 , wherein the cyclic peptide conjugate comprises the conjugate polymer and the cyclic peptide in a ratio of about 1:1 to about 4:1.
11. The method of claim 1 , further comprising coating the surface of the substrate with a coating polymer prior to contacting with the mixture, the coating polymer comprising at least one of a random copolymer of styrene (S), methylmethacrylate (MMA) and benzocyclobutene (BCB).
12. The method of claim 11 , further comprising annealing the coating polymer.
13. A method of making a porous thin film, the method comprising:
contacting a substrate with a mixture comprising a plurality of cyclic peptide conjugates each comprising a cyclic peptide and a conjugate polymer covalently linked to the cyclic peptide, and a plurality of block copolymers each block copolymer comprising a first block able to bind to a conjugate polymer of the plurality of cyclic peptide conjugates and a second block that does not substantially bind to the conjugate polymer; and
annealing the mixture, such that the plurality of cyclic peptide conjugates self-assemble vertically in horizontal planes to form a plurality of cyclic peptide nanotubes perpendicular to the substrate and extending from a top surface to a bottom surface of the porous thin film, and wherein the plurality of block copolymers are arranged in the horizontal planes and extend substantially radially from the plurality of cyclic peptides.
14. A porous thin film comprising:
a plurality of cyclic peptide conjugates each comprising a cyclic peptide and a conjugate polymer covalently linked to the cyclic peptide; and
a plurality of block copolymers each block copolymer comprising a first block able to bind to a conjugate polymer of the plurality of cyclic peptide conjugates and a second block that does not substantially bind to the conjugate polymer, wherein the plurality of cyclic peptide conjugates self-assemble vertically in horizontal planes to form a plurality of cyclic peptide nanotubes perpendicular to the substrate and extending from a top surface to a bottom surface of the porous thin film.
15. The porous thin film of claim 14 , wherein the plurality of block copolymers are arranged in the horizontal planes and extend substantially radially from the plurality of cyclic peptides.