Physicochemical (PCP) based consensus sequences and uses thereof
Provided herein is a computational method for designing a PCP-consensus protein for a family of related proteins. The method uses a consensus alignment of a protein domain common to all the related proteins, which may or may not be substantially biased, from which an average value of p, e.g., 5, physicochemical properties are calculated for each amino acid in the alignment. The PCP-consensus protein has a sequence derived from an alignment of protein domains from a family of related proteins, said sequence containing one or more motifs common to all of the proteins. Also provided are the PCP-consensus proteins, kits comprising the same, datasets of aligned consensus sequences used to derive the PCP-consensus proteins and methods of eliciting an immune response, diagnosing or treating an infectious disease using the same.
1. A physicochemical properties (PCP)-consensus protein, comprising:
an isolated polypeptide having the sequence of SEQ ID NO:329, SEQ ID NO:326, SEQ ID NO:327, or SEQ ID NO:328.
2. A physicochemical properties (PCP)-consensus protein comprising isolated polypeptide having the sequence of SEQ ID NO:328.
3. A method for inducing an immune response in a subject against one or more strains of Dengue virus, comprising:
administering an isolated polypeptide of claim 1 to a subject, wherein the subject produces an immune response to Dengue virus.
4. A kit, comprising:
one or more isolated viral physicochemical properties (PCP)-consensus proteins having the sequence of SEQ ID NO:329, SEQ ID NO:326, SEQ ID NO:327, SEQ ID NO:328, or SEQ ID NO:333;
one or more isolated antibodies directed against the viral PCP-consensus proteins; or
a combination thereof.