Method of immunotherapy for treament of human papillomavirus infection
A method of treating human papillomavirus (HPV), by administering a therapeutically effective amount of a primary cell-derived biologic to a patient infected with HPV, and inducing an immune response to HPV. A method of overcoming HPV-induced immune suppression of Langerhans cells (LC), by administering a therapeutically effective amount of a primary cell-derived biologic to a patient infected with HPV, and activating LC. A method of increasing LC migration towards lymph nodes, by administering a therapeutically effective amount of a primary cell-derived biologic to a patient infected with HPV, activating LC, and inducing LC migration towards lymph nodes. A method of generating immunity against HPV, by administering an effective amount of a primary cell derived biologic to a patient infected with HPV, generating immunity against HPV, and preventing new lesions from developing.
1. A method of immunotherapy for treating human papillomavirus (HPV) infection in a female patient, comprising:
administering an effective amount of a composition consisting of a primary cell-derived biologic and a pharmaceutically acceptable carrier to a female patient infected with HPV, such that an immune response is induced to the HPV in the patient and wherein the primary cell-derived biologic includes the cytokines interleukin (IL)-1, interleukin (IL)-2, interleukin (IL)-6, interleukin (IL)-8, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.
2. The method of claim 1 , wherein the primary cell-derived biologic includes a concentration of IL-1 from 60-6,000 pcg/mL, a concentration of IL-2 from 600-60,000 pcg/mL, a concentration of IL-6 from 60-6,000 pcg/mL, a concentration of IL-8 from 6000-600,000 pcg/mL, a concentration of TNF-α from 200-20,000 pcg/mL, and a concentration of IFN-γ from 200-20,000 pcg/mL.
3. The method of claim 1 , wherein the primary cell-derived biologic further includes interleukin (IL)-7, interleukin (IL)-12, interleukin (IL)-15, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF).
4. The method of claim 1 , wherein the method further comprises:
administering an effective amount of one or more of a chemical inhibitor, a non-steroidal anti-inflammatory drug (NSAID), and zinc to the patient.
5. The method of claim 1 , wherein the chemical inhibitor is cyclophosphamide.
6. The method of claim 4 , wherein the NSAID is indomethacin.
7. The method of claim 1 , wherein the composition is a sterile, injectable solution.
8. The method of claim 1 , wherein the administering of the composition is injection into the patient's epithelium.
9. The method of claim 1 , wherein the administering of the composition is topical application to the patient's epithelium.
10. The method of claim 1 , wherein the method is such as to prevent new lesions from developing.
11. The method of claim 1 , wherein the patient has not received prior radiation treatment.
12. A method of immunotherapy for treating human papillomavirus (HPV) infection in a patient that does not have cancer, comprising:
administering an effective amount of a composition consisting of a primary cell-derived biologic and a pharmaceutically acceptable carrier to a patient infected with HPV that does not have cancer, such that an immune response is induced to the HPV in the patient and wherein the primary cell-derived biologic includes the cytokines interleukin (IL)-1, interleukin (IL)-2, interleukin (IL)-6, interleukin (IL)-8, interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha.
13. The method of claim 12 , wherein the primary cell-derived biologic includes a concentration of IL-1 from 60-6,000 pcg/mL, a concentration of IL-2 from 600-60,000 pcg/mL, a concentration of IL-6 from 60-6,000 pcg/mL, a concentration of IL-8 from 6000-600,000 pcg/mL, a concentration of TNF-α from 200-20,000 pcg/mL, and a concentration of IFN-γ from 200-20,000 pcg/mL.
14. The method of claim 12 , wherein the primary cell-derived biologic further includes interleukin (IL)-7, interleukin (IL)-12, interleukin (IL)-15, granulocyte-macrophage colony-stimulating factor (GM-CSF), and granulocyte colony-stimulating factor (G-CSF).
15. The method of claim 12 , wherein the method further comprises:
administering an effective amount of one or more of a chemical inhibitor, a non-steroidal anti-inflammatory drug (NSAID), and zinc to the patient.
16. The method of claim 15 , wherein the chemical inhibitor is cyclophosphamide.
17. The method of claim 15 , wherein the NSAID is indomethacin.
18. The method of claim 12 , wherein the composition is a sterile, injectable solution.
19. The method of claim 12 , wherein the administering of the composition is injection into the patient's epithelium.
20. The method of claim 12 , wherein the administering of the composition is topical application to the patient's epithelium.
21. The method of claim 12 , wherein the method is such as to prevent new lesions from developing.