Dispensing nozzle for automatic analyzer, and automatic analyzer including same
View Patent ↗An automatic analyzer for analyzing samples, such as urine and blood, is adapted so that analysis measured values are not affected by carry over by a repeatedly used dispensing nozzle. Coating the surface of the dispensing nozzle with a chemisorbed polyethylene glycol derivative forms a molecular layer that inhibits the adsorption of biopolymers, to reduce carry over by the dispensing nozzle.
1. An automatic analyzer comprising:
a plurality of sample containers each containing a sample;
a plurality of reagent containers each containing a reagent;
a plurality of reaction Cells into which the samples and the reagents are to be injected;
a sample dispensing mechanism comprising a sample dispensing nozzle and dispensing the samples in the sample containers into the reaction Cells;
a reagent dispensing mechanism comprising a reagent dispensing nozzle and dispensing the reagents in the reagent containers into the reaction Cells, and
electrostatic capacitance measuring means for measuring electrostatic capacitance between the sample dispensing nozzle and the reaction Cell, wherein
the sample dispensing nozzle has a nozzle main body made of metal, and a silicon oxide layer on an outer surface of the nozzle main body, and a silicon derivative having polyethylene glycol, represented by the following general formula:
Si—R 1 —(OCH 2 CH 2 ) n —O—R 2 (n is a positive integer of 2 or more, R 1 is a hydrocarbon group, and R 2 is H or CH 3 )
is chemisorbed on the silicon oxide layer, and
the electrostatic capacitance measuring means measures the electrostatic capacitance via the silicon oxide layer and the silicon derivative.
2. The automatic analyzer according to claim 1 , comprising a mechanism for detecting an abnormality of the silicon oxide layer on the sample dispensing nozzle surface based on a change in the electrostatic capacitance; and an indicator for indicating the abnormality on detection of the abnormality.
3. The automatic analyzer according to claim 1 , wherein a region of the sample dispensing nozzle where the polyethylene glycol derivative is chemisorbed is larger than a region where the sample dispensing nozzle is immersed in the sample during dispensing operation.
4. The automatic analyzer according to claim 1 , comprising a mechanism for chemisorbing the polyethylene glycol derivative on the sample dispensing nozzle.
5. The automatic analyzer according to claim 1 , wherein the polyethylene glycol derivative is a 2-methoxypolyethyleneoxysilane derivative.
6. A method for manufacturing a dispensing nozzle for the automatic analyzer of claim 1 used for dispensing a sample in a sample container into a reaction cell and measuring distance from the sample in the reaction cell by electrostatic capacitance detection, comprising the steps of:
forming a silicon oxide layer on a surface of a metal nozzle using sputtering or drug solution application and drying;
washing the silicon oxide layer formed on the surface of the metal nozzle;
immersing the washed dispensing nozzle in a solution of a polyethylene glycol derivative having a silanol group precursor, represented by the following general formula:
R 1 R 2 R 3 Si—R 4 —(OCH 2 CH 2 ) n —O—R 5 (R 1 , R 2 , and R 3 are substituents on silicon, R 4 is a hydrocarbon group, R 5 is H or CH 3 , and n is a positive integer of 2 or more);
washing the treated surface of the metal nozzle with a solvent; and drying the washed surface of the dispensing nozzle.