Chromatographic method for purifying FC-containing proteins
The present invention relates to methods of depleting impurities, in particular host cell proteins (HCP) and DNA from cell culture supernatants by means of protein A chromatography using a novel washing buffer.
1. A method of depleting impurities from a composition that contains a protein which comprises the Fc domain of an immunoglobulin (target protein), by protein-A chromatography, comprising the steps of:
a. applying a mobile phase which contains the target protein to a stationary phase which contains protein A, under conditions in which the target protein binds to the stationary phase;
b. applying a washing buffer with a pH of between 4 and 8 as mobile phase, containing
i. arginine in a concentration of 0.1-1 mol/l,
ii. sodium chloride in a concentration of 0.2 to 2 mol/l,
iii. an alcohol selected from among isopropanol, n-propanol and ethanol, in a concentration of 5-30% (w/v) and
iv. polyvinylpyrrolidone in a concentration of 0.05-2% (w/v);
c. using an elution buffer as mobile phase under conditions in which the target protein is eluted from the stationary phase.
2. The method according to claim 1 , wherein the arginine concentration in the washing buffer is 0.4-0.6 mol/l.
3. The method according to claim 1 , wherein the sodium chloride concentration in the washing buffer is 0.9-1.1 mol/l.
4. The method according to claim 1 , wherein the alcohol in the washing buffer is isopropanol in a concentration of 10-20% (w/v).
5. The method according to claim 1 , wherein polyvinylpyrrolidone (PVP) is in a concentration of 0.1-2% (w/v).
6. The method according to claim 1 , wherein the impurities are host cell proteins (HCP).
7. A method of depleting impurities from a composition that contains a protein which comprises the Fc domain of an immunoglobulin (target protein), by protein-A chromatography, comprising the steps of:
a. applying a mobile phase which contains the target protein to a stationary phase which contains protein A, under conditions in which the target protein binds to the stationary phase;
b. applying a washing buffer with a pH of between 5 and 8 as mobile phase, containing
i. arginine in a concentration of 0.1—1 mol/l,
ii. sodium chloride in a concentration of 0.2 to 2 mol/l,
iii. an alcohol selected from among isopropanol, n-propanol and ethanol, in a concentration of 5—30% (w/v) and
iv. polyvinylpyrrolidone in a concentration of 0.05—2% (w/v);
c. using an elution buffer as mobile phase under conditions in which the target protein is eluted from the stationary phase.
8. The method according to claim 7 , wherein the arginine concentration in the washing buffer is 0.4—0.6 mol/l.
9. The method according to claim 7 , wherein the sodium chloride concentration in the washing buffer is 0.9—1.1 mol/l.
10. The method according to claim 7 , wherein the alcohol in the washing buffer is isopropanol in a concentration of 10—20% (w/v).
11. The method according to claim 7 , wherein polyvinylpyrrolidone (PVP) is in a concentration of 0.1—2% (w/v).
12. The method according to claim 7 , wherein the impurities are host cell proteins (HCP).