Characterization of granulocytic Ehrlichia and methods of use
The present invention relates, in general, to methods of screening a sample obtained from a subject for antibodies relating to granulocytic ehrlichia (GE) infection.
1. A method of screening a test sample obtained from a subject for antibodies relating to granulocytic Ehrlichiosis infection comprising:
(a) providing one or more purified polypeptides that bind to an antibody that recognizes an immunologically reactive fragment of an amino acid sequence selected from the group consisting of SEQ ID NO: 4, 6, 2, 8, 21, 22, 39, 27, 29 and 30;
(b) providing the test sample;
(c) contacting the sample with said one or more polypeptides under conditions that allow immunocomplexes to form, wherein the immunocomplexes comprise said one or more purified polypeptides bound to the antibody; and
(d) detecting the presence or absence of the immunocomplexes.
2. The method according to claim 1 , wherein the test sample is obtained from a dog.
3. The method according to claim 1 , wherein the test sample is a blood sample.
4. The method according to claim 3 , wherein the blood sample is a serum sample.
5. The method according to claim 1 , wherein the step of detecting is carried out using an immunofluorescence assay.
6. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 4.
7. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 6.
8. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 2.
9. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 8.
10. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 21.
11. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 22.
12. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 39.
13. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 27.
14. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 29.
15. The method according to claim 1 , wherein the antibody recognizes an immunologically reactive fragment of an amino acid sequence set forth as SEQ ID NO: 30.