Kit for predicting implantation success in assisted fertilization
A kit for determining for a female subject the implantation potential of embryos obtained or to be obtained by assisted fertilization is described. The kit includes at least one reagent suitable for detection of levels of FF G-CSF or FF G-CSF mRNA, such as anti-G-CSF antibody or a nucleic acid probe for detection of levels of G-CSF mRNA. The kit may also include a set of concentration standards of FF G-CSF and aspirator tips for removing an oocyte and follicular fluid.
1. A kit for determining for a female subject the implantation potential of embryos obtained or to be obtained by assisted fertilization comprising:
system for measuring for a plurality of oocytes collected from said subject the level of follicular fluid granulocyte-colony stimulating factor (G-CSF) present in the follicular fluid (FF) of a follicle of each collected oocyte, which system comprises at least one reagent suitable for detection of levels of FF G-CSF or FF G-CSF mRNA, and
a plurality of disposable aspirator tips for removing an oocyte and follicular fluid from a subject.
2. Kit according claim 1 , further comprising a set of concentration standards of G-CSF.
3. Kit according claim 2 , wherein the concentration standards are commercial G-CSF standards.
4. Kit according to claim 1 , wherein the at least one reagent comprises an anti-G-CSF antibody for detection of levels of FF G-CSF.
5. Kit according to claim 4 , wherein the anti-G-CSF antibody is labeled.
6. Kit according to claim 1 , wherein the reagent is a nucleic acid probe for detection of levels of G-CSF mRNA.
7. Kit according to claim 6 , wherein the nucleic acid probe is labeled.