Process for preparing diaminophenothiazinium compounds
Process for preparing compounds of the diaminophenothiazinium type of formula (II) below. The products have a high degree of purity and are useful for the preparation of medicaments. In which the R 1 , R 2 , R 3 , R 4 , R 5 , R 6 , R 7 , R 8 , R 9 and R 10 groups having the same definition as in formula (I), and R representing a group chosen from: a phenyl or benzyl group, optionally substituted with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group, a linear, branched or cyclic C 1 -C 8 alkyl group, a C 1 -C 8 alkylamino group, a C 1 -C 8 alkoxy group, a phenyloxy or benzyloxy group optionally substituted on the aromatic nucleus with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group, Z representing an atom chosen from O and S.
1. A methylene blue (3,7-bis(dimethylamino)phenothiazin-5-ylium chloride) comprising less than 3% of impurities measured by HPLC under the conditions of the European Pharmacopeia 5.4 and less than 20 μg/g of total metal content.
2. A methylene blue according to claim 1 wherein it comprises less than 15 μg/g of total metal content.
3. A methylene blue according to claim 1 wherein it comprises less than 10 μg/g of total metal content.
4. A methylene blue according to claim 1 , wherein it comprises 0.02 μg or less of cadmium per g of methylene blue.
5. A methylene blue according to claim 1 , wherein it comprises less than 4.5 μg/g of aluminum.
6. A methylene blue according to claim 1 , wherein it comprises less than 2.5 μg/g of aluminum.
7. A methylene blue according to claim 1 , wherein it comprises less than 0.5 g/g of tin.
8. A methylene blue according to claim 1 , wherein it comprises less than 0.3 μg/g of tin.
9. A methylene blue according to claim 1 , wherein it comprises 0.95 μg or less of chromium per g of methylene blue.
10. A methylene blue according to claim 1 , wherein it is obtained by a method which comprises at least one step during which a compound of formula (II):
in which
each of R 1 , R 2 , R 3 , R 4 represent a methyl group,
each of R 5 , R 6 , R 7 , R 8 , R 9 and R 10 represent a hydrogen atom,
R represents a group chosen from:
a phenyl or benzyl group, optionally substituted with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group,
a phenyloxy or benzyloxy group optionally substituted on the aromatic nucleus with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group,
Z represents an atom chosen from O and S,
is subjected to a purification step under conditions which make it possible to separate metal compounds from the compounds of formula (II), wherein the purification step comprises at least one filtration through a support capable of retaining the metal compounds, and the filtration support is chosen from: a silica gel, a neutral, basic or acidic alumina gel, a microporous membrane, a resin grafted with metal-capturing groups, and fibers grafted with metal-capturing groups,
and said method further comprises at least one step during which the compound of formula (II) is subjected to a step for deprotection of the R—CZ group from the amine of the phenothiazine ring.
11. A methylene blue according to claim 10 , wherein in formula II Z represents O and R represents a phenyl.
12. A methylene blue according to claim 1 , wherein it is obtained by a method which comprises at least one step during which a compound of formula (II):
in which
each of R 1 , R 2 , R 3 , R 4 represent a methyl group,
each of R 5 , R 6 , R 7 , R 8 , R 9 and R 10 represent a hydrogen atom,
R represents a group chosen from:
a phenyl or benzyl group, optionally substituted with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group,
a phenyloxy or benzyloxy group optionally substituted on the aromatic nucleus with one or more functions chosen from: a C 1 -C 4 alkyl, a halogen atom, a C 1 -C 4 haloalkyl and a nitro group,
Z represents an atom chosen from O and S,
is subjected to a purification step under conditions which make it possible to separate metal compounds from the compounds of formula (II), wherein the purification step comprises at least one filtration through a support capable of retaining the metal compounds, and the filtration support is chosen from: a silica gel, a neutral, basic or acidic alumina gel, a microporous membrane, a resin grafted with metal-capturing groups, and fibers grafted with metal-capturing groups, and wherein said method further comprises at least one step wherein the compound of formula (II) is subjected to a step for deprotection of the R—CZ group from the amine of the phenothiazine ring using deprotection means chosen from: quinones.
13. A methylene blue according to claim 12 , wherein the deprotection means is 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ).
14. A medicament comprising a methylene blue as claimed in claim 1 , in a pharmaceutically acceptable carrier.