Interleukin-10 antibodies
View Patent ↗The methods and compositions provided herein relate generally to IL-10 specific antibodies and uses thereof. More specifically, compositions of humanized IL-10 specific antibodies and methods to use such antibodies in modulating the biological activity of IL-10, particularly in autoimmune disorders and pathogen-mediated immunopathology.
1. An isolated nucleic acid sequence encoding a humanized antibody, or antigen binding fragment thereof, that binds IL-10, wherein the humanized antibody or antigen binding fragment thereof comprises:
a) at least one light chain variable region having the amino acid sequence of SEQ ID NO: 4; or
b) at least one heavy chain variable region having the amino acid sequence of SEQ ID NO: 9.
2. An expression vector comprising the isolated nucleic acid of claim 1 operably linked to control sequences recognized by a host cell transfected with the vector.
3. A host cell comprising the vector of claim 2 .
4. The nucleic acid of claim 1 , wherein the light chain comprises the nucleic acid sequence of SEQ ID NO: 19 and the heavy chain comprises the nucleic acid sequence of SEQ ID NO: 20.
5. An expression vector comprising the nucleic acid of claim 4 operably linked to control sequences recognized by a host cell transfected with the vector.
6. A host cell comprising the vector of claim 5 .
7. The nucleic acid of claim 1 , wherein the antigen binding fragment is selected from the group consisting of a Fab, Fab′, Fab′-SH, Fv, sc-Fv, F(ab)2 and a diabody.