Process for the purification of high-purity Rubusoside
The invention provides a process of purifying Rubusoside from the Rubus suavissimus S. Lee plant extract. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakeries, cookies, chewing gums, and alike.
1. A process for purifying Rubusoside from Rubus suavissimus extract comprising the steps of:
a. providing an extract of Rubus suavissimus S. Lee leaves;
b. dissolving the extract in a first aqueous alcoholic solution to obtain a first mixture;
c. inducing crystallization in the first mixture by temperature gradient treatment;
d. filtering the first mixture from (c) to obtain a first precipitate and a first filtrate;
e. dissolving the first precipitate in a second aqueous alcoholic solution to obtain a second mixture;
f. adding activated carbon to second mixture and filtering it to obtain a decolorized second mixture;
g. inducing crystallization in the decolorized second mixture by temperature gradient treatment;
h. filtering the decolorized second mixture from (g) to obtain a second precipitate and a second filtrate;
i. suspending the second precipitate in a third aqueous alcoholic solution to obtain a third mixture;
j. filtering the third mixture to obtain a third precipitate and a third filtrate; and
k. drying the third precipitate to yield purified Rubusoside.
2. The process of claim 1 wherein the first aqueous alcoholic solution in step (b) is a methanol-water solution, with 75-99% methanol.
3. The process of claim 1 wherein the second aqueous alcoholic solution in step (e) is a methanol-water solution with 70-90% methanol.
4. The process of claim 1 wherein the third aqueous alcoholic solution in step (i) is a methanol-water solution with 90-99% methanol.
5. The process of claim 1 wherein in steps (c) and (g) the temperature gradient treatment inducing crystallization comprises increasing the mixture temperature to 65-70° C. then gradually cooling it to 10° C. at a rate of 10° C. per hour with continuous mild agitation.
6. The process of claim 1 wherein the aqueous alcoholic solutions comprises one or more organic solvents selected from the group consisting of methanol, ethanol, 1-propanol, and isopropanol.
7. The process of claim 1 , wherein the purified Rubusoside has a purity greater than 95% on a dry basis.
8. The process of claim 1 , wherein the purified Rubusoside has a purity greater than 98% on a dry basis.