Molecular engineering of a floral inducer for crop improvement
A plant comprising a modified Flowering Locus (FT) polynucleotide expressing a modified polypeptide exhibits altered flowering time, floral numbers and/or increased seed production. Different mutant sequences conferring different phenotypes are disclosed.
1. A modified plant comprising and expressing a modified Flowering Locus T (FT) polynucleotide, said polynucleotide encoding a modified polypeptide comprising an amino acid modification in SEQ ID NO: 2, wherein said plant exhibits increased fruit and/or seed production, and wherein said modification comprises an amino acid substitution at V70 and/or an amino acid substitution at T27.
2. A plant according to claim 1 , wherein the amino acid substitution is with a neutral amino acid.
3. A plant according to claim 2 , wherein the amino acid substitution is with Alanine.
4. A plant according to claim 1 , wherein the FT polynucleotide is operably linked to a regulatory sequence.
5. A plant according to claim 4 , wherein said regulatory sequence is a promoter.
6. A plant according to claim 1 , wherein the plant is a monocot or dicot plant.
7. A plant according to claim 6 , wherein said plant is a crop plant.
8. A plant according to claim 7 , wherein said plant is selected from the group consisting of maize, wheat, rice, sorghum, soybean, sugar cane, sugar beet, forage grass, turfgrass, switchgrass, miscanthus, potato, tomato, barley, pea, bean, field bean, cotton, lettuce, sunflower, brassicas, canola, oilseed rape, broccoli, alfalfa, and poplar.
9. A plant according to claim 1 , wherein said modified FT polynucleotide is a modified endogenous polynucleotide.
10. A plant according to claim 1 , wherein said modified FT polynucleotide is a modified exogenous polynucleotide.
11. Plant tissue or harvestable material derived from a plant according to claim 1 .
12. An isolated FT nucleic acid, said nucleic acid encoding a modified polypeptide comprising an amino acid modification wherein said modification results in an altered phenotype, when said nucleic acid is introduced in a plant wherein said phenotypes is increased flowering and increased fruit and/or seed production; and wherein said modification comprises or consists of an amino acid substitution in SEQ ID NO: 2 at V70 and/or an amino acid substitution at T27.
13. An isolated nucleic acid according to claim 12 wherein the amino acid is substituted with A.
14. A vector comprising an isolated FT nucleic acid as defined in claim 12 .
15. A plant comprising a nucleic acid as defined in claim 12 or a vector as defined in claim 14 .