IP Library › Granted Patent US 8,951,532
Granted Patent B2
US 8,951,532 · App. 13/575,217 · Granted Feb 10, 2015

A25 bacteriophage lysin

Inventors: Brion Mermer (Gray, ME); William J. Palin (Cape Elizabeth, ME); Nancy Turcotte (Auburn, ME)
Assignee: Alere Scarborough, Inc.
G01N33/56944C07K14/005C12N1/06C12N2795/00022G01N2800/26
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Quick Facts
Patent No.
US 8,951,532
App. No.
13/575,217
Granted
Feb 10, 2015
Kind
B2
Abstract

The invention relates to the identification, sequencing, and isolation of an A25 bacteriophage lysin gene that expresses a protein involved in the lysis of bacterial cells during the phage life cycle. The invention further relates to methods for lysing certain bacteria using lysin, which are useful for example in a diagnostic procedure designed to detect these bacteria.

Claims (6)

1. An isolated fusion polypeptide comprising an amino acid sequence with at least 85% sequence identity to SEQ ID NO:2 and a purification tag.

2. The isolated fusion polypeptide of claim 1 , comprising an amino acid sequence with at least 90% sequence identity to SEQ ID NO:2.

3. The isolated fusion polypeptide of claim 1 , comprising an amino acid sequence with at least 95% sequence identity to SEQ ID NO:2.

4. The isolated fusion polypeptide of claim 1 , comprising an amino acid sequence with at least 99% sequence identity to SEQ ID NO:2.

5. An isolated fusion polypeptide comprising the amino acid sequence of SEQ ID NO:2 and a purification tag.

6. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a therapeutically effective amount of an isolated fusion polypeptide comprising the amino acid sequence of SEQ ID NO:2 and a purification tag.

Assignments (5)
CHANGE OF NAME Recorded Jan 8, 2020
From: ALERE SCARBOROUGH, INC.
To: ABBOTT DIAGNOSTICS SCARBOROUGH, INC.
Reel/Frame 051504/0797 →
RELEASE OF SECURITY INTEREST IN INTELLECTUAL PROPERTY RECORDED AT REEL 036994, FRAME 0192 AND REEL 037115, FRAME 0498 Recorded Oct 5, 2017
From: HEALTHCARE FINANCIAL SOLUTIONS, LLC, AS COLLATERAL AGENT
To: ALERE CONNECT, LLC; ALERE SAN DIEGO, INC. (FKA BIOSITE INC. OR FKA CHOLESTECH CORP. OR FKA HEMOSENSE INC. OR FKA INVERNESS MEDICAL-BIOSTAR INC. OR FKA ISCHEMIA TECHNOLOGIES, INC. OR FKA TWISTDX, INC.); ALERE SCARBOROUGH, INC. (FKA MATRITECH, INC. FKA ADVANTAGE DIAGNOSTICS CORP. OR FKA BINAX, INC. OR FKA MILANO ACQUISITION CORP.); INNOVACON, INC. (FKA APPLIED BIOTECH, INC. OR FKA AMEDITECH INC.); IONIAN TECHNOLOGIES, LLC (FKA IONIAN TECHNOLOGIES, INC.); QUALITY ASSURED SERVICES INC. (FKA ZYCARE INC.); STANDING STONE, LLC; ESCREEN, INC.
Reel/Frame 044213/0258 →
ASSIGNMENT OF IP SECURITY AGREEMENT, PREVIOUSLY RECORDED AT REEL 036994, FRAME 0192 Recorded Nov 16, 2015
From: GENERAL ELECTRIC CAPITAL CORPORATION, AS RETIRING ADMINISTRATIVE AGENT
To: HEALTHCARE FINANCIAL SOLUTIONS, LLC, AS SUCCESSOR ADMINISTRATIVE AGENT
Reel/Frame 037115/0498 →
INTELLECTUAL PROPERTY SECURITY AGREEMENT Recorded Oct 29, 2015
From: ALERE CONNECT, LLC; ALERE SAN DIEGO, INC. (FKA BIOSITE INC. OR FKA CHOLESTECH CORP. OR FKA HEMOSENSE INC. OR FKA INVERNESS MEDICAL-BIOSTAR INC. OR FKA ISCHEMIA TECHNOLOGIES, INC. OR FKA TWISTDX, INC.); ALERE SCARBOROUGH, INC. (FKA MATRITECH, INC. FKA ADVANTAGE DIAGNOSTICS CORP. OR FKA BINAX, INC. OR FKA MILANO ACQUISITION CORP.); INNOVACON, INC. (FKA APPLIED BIOTECH, INC. OR FKA AMEDITECH INC.); IONIAN TECHNOLOGIES, LLC (FKA IONIAN TECHNOLOGIES, INC.); QUALITY ASSURED SERVICES INC. (FKA ZYCARE INC.); STANDING STONE, LLC; ESCREEN, INC.
To: GENERAL ELECTRIC CAPITAL CORPORATION, AS COLLATERAL AGENT
Reel/Frame 036994/0192 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 9, 2012
From: MERMER, BRION; PALIN, WILLIAM J.; TURCOTTE, NANCY
To: ALERE SCARBOROUGH, INC.
Reel/Frame 029272/0353 →
Continuity (2)
Provisional Application 61298039 · Jan 25, 2010
Related Publication 20130029351A1 · Jan 31, 2013