Nucleic acid analyzer, reaction device for nucleic acid analysis and substrate of reaction device for nucleic acid analysis
View Patent ↗Provided is a reaction device for nucleic acid analysis wherein microparticles, which carry a nucleic acid to be detected having been immobilized thereon, are aligned in a lattice form on a substrate according to the pixel size of a two-dimensional sensor. By this reaction device for nucleic acid analysis which is provided with a channel-forming reaction chamber on the substrate ( 101 ), the nucleic acid having been immobilized on the microparticles ( 103 ) on the substrate ( 101 ) is detected. The microparticles ( 103 ), which carry the nucleic acid to be detected having been immobilized thereon, are arranged by microstructures ( 102 ) aligned on the substrate ( 101 ).
1. A reaction device for nucleic acid analysis which detects nucleic acids, comprising:
a substrate;
a carrier disposed on the substrate, wherein the nucleic acids are immobilized on the carrier; and
a reaction chamber, having a flow channel, disposed on the substrate, the reaction chamber having a shape of a channel;
wherein microstructures in the form of protrusions are arranged regularly on the substrate within the reaction chamber, the microstructures comprising a first microstructure; and four second microstructures which are positioned in a rectangular pattern and are taller in height of protrusions from the substrate than the first microstructure, the first microstructure being provided at a center of the rectangular pattern of the four second microstructures,
wherein the carrier is immobilized by the microstructures so that a bottom part of the carrier is spaced apart from the substrate; and
wherein the carrier can be immobilized in contact with the first microstructure and the four second microstructures.
2. The reaction device for nucleic acid analysis according to claim 1 , wherein the microstructures are arranged in a lattice form.
3. The reaction device for nucleic acid analysis according to claim 1 , wherein a functional group for capturing the carrier is introduced to the substrate.
4. The reaction device for nucleic acid analysis according to claim 1 , wherein the carrier comprises a magnetic material.
5. The reaction device for nucleic acid analysis according to claim 1 , wherein the carrier is microparticle.
6. A substrate for a reaction device for nucleic acid analysis used to detect nucleic acids, comprising:
a carrier disposed on the substrate, wherein the nucleic acids are immobilized on the carrier; and
microstructures in the form of protrusions regularly arranged on the substrate, the microstructures comprising a first microstructure; and four second microstructures which are positioned in a rectangular pattern and are taller height of protrusions from the substrate than the first microstructure, the first microstructure being provided at a center of the rectangular pattern of the four second microstructures;
wherein the carrier can be immobilized in contact with the first microstructure and the four second microstructures.
7. The substrate for a reaction device for nucleic acid analysis according to claim 6 , wherein the microstructures are arranged in a lattice form.