miRNA in the diagnosis of ovarian cancer
The present invention provides novel methods for diagnosing a state of health based on the determination of specific miRNAs that have altered expression levels in different conditions, e.g. disease states compared to healthy controls.
1. A method of detecting a miRNA expression profile in a human subject, the method comprising:
(a) providing a blood cell sample comprising erythrocytes, leukocytes, and thrombocytes from a human subject;
(b) detecting the expression level of an miRNA set consisting of hsa-miR-342-3p (SEQ ID NO: 442) and hsa-miR-146a (SEQ ID NO: 709) in the blood cell sample using an RT-PCR assay.
2. The method of claim 1 , wherein step (b) comprises:
(i) isolating RNA from the blood cell sample;
(ii) amplifying the isolated RNA to produce an amplified nucleic acid sample;
(iii) contacting the amplified nucleic acid sample with a first probe complementary to hsa-miR-342-3p (SEQ ID NO: 442) and a second probe complementary to hsa-miR-146a (SEQ ID NO: 709); and
(iv) detecting binding of the first and second probes.
3. The method of claim 1 , wherein step (a) comprises obtaining a blood sample from the human subject and isolating a cellular fraction from the blood sample, thereby producing blood cell sample.
4. The method of claim 1 , wherein the human subject is a subject suspected of having ovarian cancer.