Telomerase polypeptide vaccine for treating cancer
A polypeptide comprising the sequence of SEQ. ID NO. 2, 3, 4, 7 or 8. The polypeptide may have the sequence of an immunogenic fragment thereof comprising at least eight amino acids, wherein the immunogenic fragment is not one of SEQ. ID NOS. 6 or 11 to 16. The polypeptide may have a sequence having at least 80% sequence identity to the aforementioned polypeptide or immunogenic fragment. The polypeptide is less than 100 amino acids in length and does not comprise the sequence of any of SEQ. ID NOS. 10, 46, 56, 57 or 59 to 62 and does not consist of the sequence of SEQ ID NO. 58. The polypeptide is useful in the treatment or prophylaxis of cancer.
1. A polypeptide selected from the group consisting of:
i) a polypeptide comprising the sequence of SEQ ID NO:1, wherein the polypeptide is less than 100 amino acids in length;
ii) a polypeptide comprising the sequence of an immunogenic fragment of i) comprising at least 24 amino acids, wherein the polypeptide is less than 100 amino acids in length;
iii) a polypeptide consisting of the sequence of an immunogenic fragment of i), wherein the immunogenic fragment consists of the sequence of any one of SEQ ID NOS: 18, 24, 36 or 37; and
iv) a sequence having at least 95% sequence identity to i), ii) or iii), wherein the polypeptide of i), ii), iii) or iv) is less than 100 amino acids in length and wherein the polypeptide does not comprise the sequence of any of SEQ ID NOS: 10, 46, 57 or 59 to 62 and does not consist of the sequence of SEQ ID NO: 58.
2. A polypeptide according to claim 1 wherein the polypeptide is less than 11, 20, 30, 50 or 80 amino acids in length.
3. A pharmaceutical composition comprising the polypeptide according claim 1 and a pharmaceutically acceptable adjuvant, diluent or excipient and wherein the composition optionally includes another therapeutic ingredient.
4. A pharmaceutical composition according to claim 3 wherein the polypeptide is present in a dose of between 50 and 200 μg.
5. The pharmaceutical composition of claim 3 , wherein the composition comprises an adjuvant selected from the group consisting of Freund's complete adjuvant, Freund's incomplete adjuvant, aluminum phosphate, aluminum hydroxide, alum, cholera toxin, salmonella toxin, granulocyte macrophage colony stimulating factor (GM-CSF), and a combination thereof.
6. The pharmaceutical composition of claim 5 , wherein the polypeptide is coupled to an immunogenic carrier selected from the group consisting of keyhole limpet haemocyanin, bovine serum albumin, ovalbumin and fowl immunoglobulin.
7. A cocktail of polypeptides comprising at least first and second different polypeptides wherein the first polypeptide is a polypeptide according to claim 1 and wherein the second polypeptide comprises a sequence selected from the group consisting of:
i) SEQ ID NOS: 2 to 7;
ii) the sequence of an immunogenic fragment of i) comprising at least eight amino acids; and
iii) a sequence having at least 80% sequence identity to i) or ii), wherein the second polypeptide is less than 100 amino acids in length.
8. A cocktail of polypeptides according to claim 7 wherein the second polypeptide comprises a sequence selected from the group consisting of:
i) SEQ ID NOS: 1, 7, 8, 9 or 10;
ii) the sequence of an immunogenic fragment of i) comprising at least eight amino acids; and
iii) a sequence having at least 80% sequence identity to i) or ii), wherein the second polypeptide is less than 100 amino acids in length.
9. A cocktail of polypeptides according to claim 7 wherein the at least first and second different polypeptides comprise a cocktail of polypeptides selected from the group consisting of:
i) a cocktail of: a polypeptide according to claim 3 ; a polypeptide comprising the sequence of SEQ ID NO: 7 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEQ ID NO: 9 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids;
ii) a cocktail of: a polypeptide according to claim 2 ; a polypeptide comprising the sequence of SEQ ID NO: 8 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEQ ID NO: 9 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids; and
iii) a cocktail of: a polypeptide according to claim 1 ; a polypeptide comprising the sequence of SEQ ID NO: 8 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEQ ID NO: 10 or a sequence having at least 80% sequence identity thereto or an immunogenic fragment thereof comprising at least eight amino acids,
wherein each polypeptide is less than 100 amino acids in length.
10. A cocktail of polypeptides according to claim 7 wherein the or each immunogenic fragment has a sequence of any one of SEQ ID NOS: 17 to 40.
11. A polypeptide comprising the sequence of SEQ ID NO: 1, wherein the polypeptide is less than 100 amino acids in length and wherein the polypeptide does not comprise the sequence of any of SEQ ID NOS: 10, 46, 57 or 59 to 62.
12. A polypeptide according to claim 11 , wherein the polypeptide is less than 50 or 80 amino acids in length.
13. A pharmaceutical composition comprising the polypeptide of claim 11 and a pharmaceutically acceptable adjuvant, diluent or excipient and wherein the composition optionally includes another therapeutic ingredient.
14. A pharmaceutical composition according to claim 13 , wherein the polypeptide is present in a dose of between 50 and 200 μg.