Luminescent gold nanomaterial functionalized by N-(4-aminobutyl)-N-ethylisoluminol, preparation and application thereof
View Patent ↗Provided is luminescent gold nanomaterial functionalized by N-(4-aminobutyl)-N-ethylisoluminol, methods of preparation and application thereof. The functionalized gold nanomaterial is formed by N-(4-aminobutyl)-N-ethylisoluminol bonding to the surface of the gold nanoparticle. The functionalized gold nanomaterial are prepared by directly reducing chloroauric acid with N-(4-aminobutyl)-N-ethylisoluminol, wherein N-(4-aminobutyl)-N-ethylisoluminol acts as reducer and stabilizer simultaneously. The preparation method is simple, fast and no need of special conditions. The preparation methods can be performed in a wide temperature range, for example, 15-35.degree. C. The size and pattern of the functionalized gold nanomaterial can be specified by choosing the ratio of chloroauric acid to N-(4-aminobutyl)-N-ethylisoluminol. The obtained functionalized gold nanomaterial exhibits excellent chemiluminescence properties. Said functionalized gold nanomaterial can be combined with biomolecules to form biomolecular probe, which can be used for immunoassay, nucleic acid analysis, molecular imaging, sensor, etc., and has a broad application prospect in the field of clinical analysis biomedicine, food safety, and environment monitoring.
1. A functionalized gold nanomaterial comprising a gold nanoparticle and N-(4-aminobutyl)-N-ethylisoluminol molecules, said N-(4-aminobutyl)-N-ethylisoluminol molecules are connected to the surface of the gold nanoparticle directly by Au—N covalent bond.
2. The functionalized gold nanomaterial as in claim 1 is capable of reacting with oxidants and generating chemiluminescence.
3. The functionalized gold nanomaterial of claim 2 , wherein the oxidant is H 2 O 2 , O 2 , CIO − , I 2 , IO 4 − , or MnO 4 .
4. A method of synthesizing a functionalized gold nanomaterial, comprising the following steps:
(1) mixing chloroauric acid aqueous solution A with an N-(4-aminobutyl)-N-ethylisoluminol aqueous solution under stirring to get a mixture; wherein the molar concentration of said chloroauric acid in the aqueous solution A is 1-10 mmol/L and the molar concentration of N-(4-aminobutyl)-N-ethylisoluminol is 0.5-8.0 mmol/L;
(2) adding chloroauric acid aqueous solution B into the mixture obtained from said step (1) while stirring continuously until a gold colloid is formed, resulting in the functionalized gold nanomaterial comprising a gold nanoparticle and N-(4-aminobutyl)-N-ethylisoluminol molecules, wherein said N-(4-aminobutyl)-N-ethylisoluminol molecules are connected directly to the surface of the gold nanoparticle by —Au—N covalent bond; wherein the molar concentration of said chloroauric acid in the added aqueous solution B is 1-10 mmol/L.
5. The method of synthesizing the functionalized gold nanomaterial of claim 4 , wherein the molar concentration of said chloroauric acid aqueous solution added in the step (1) is 2 to 4 times more than the molar concentration of N-(4-aminobutyl)-N-ethylisoluminol wherein the molar concentration of the chloroauric acid aqueous solution B added in the step (2) is 1 to 3 times more than the amount molar concentration of N-(4-aminobutyl)-N-ethylisoluminol.
6. The method of synthesizing the functionalized gold nanomaterial according to claim 4 , wherein the molar concentration ratio between said chloroauric acid aqueous solution and N-(4-aminobutyl)-N-ethylisoluminol aqueous solution added in step (1) (Ratio 1), and the molar concentration ratio between said chloroauric acid aqueous solution and N-(4-aminobutyl)-N-ethylisoluminol aqueous solution added in step (2) (Ratio 2) are selected from the group consisting of h1, h2, h3, h4, h5, h6, h7, h8, h9, and h10, wherein:
Ratio 1
Ratio 2
h1
4
1
h2
2
3
h3
2.7
1.8
h4
2.3
1.4
h5
2.7
2.1
h6
2.7
2.4
h7
2.7
2.7
h8
3
2
h9
27/11
18/11
h10
2.25
1.5.
7. The method of synthesizing the functionalized gold nanomaterial as in claim 4 , further comprising a step of purifying gold colloid with centrifugation.
8. The method of synthesizing the functionalized gold nanomaterial of claim 7 , wherein said centrifugation is centrifuging the gold colloid under 17120*g for 45 minutes.
9. The method of synthesizing the functionalized gold nanomaterial as in claim 4 , wherein the temperature in the method step (1) and step (2) is in a range of 15-35° C.
10. The method of synthesizing the functionalized gold nanomaterial as in claim 4 , wherein the processing time of method step (1) is at least 2 hours, and step (2) is at least 1.5 hours.
11. A functionalized gold nanomaterial, wherein the gold nanomaterial is synthesized by
(1) mixing chloroauric acid aqueous solution A with an N-(4-aminobutyl)-N-ethylisoluminol aqueous solution under stirring to get a mixture; wherein the molar concentration of said chloroauric acid in the aqueous solution A is 1-10 mmol/L and the molar concentration of N-(4-aminobutyl)-N-ethylisoluminol is 0.5-8.0 mmol/L;
(2) adding chloroauric acid aqueous solution B into the mixture obtained from the step (1) while stirring continuously until a gold colloid is formed, resulting in the functionalized gold nanomaterial comprising a gold nanoparticle and N-(4-aminobutyl)-N-ethylisoluminol molecules, wherein said N-(4-aminobutyl)-N-ethylisoluminol molecules are connected to the surface of the gold nanoparticle directly by Au—N covalent bond; wherein the molar concentration of said chloroauric acid in the added aqueous solution B is 1-10 mmol/L.
12. The functionalized gold nanomaterial as in claim 1 , wherein the gold nanoparticle is characterized by morphology selected from the group consisting of spherical disperse gold nanoparticle, spherical gold nanoparticle assembled chains and spherical gold nanoparticle assembled networks.
13. The functionalized gold nanomaterial as in claim 12 , wherein the size of the spherical gold nanoparticle is 10-30 nm.
14. The functionalized gold nanomaterial as in claim 12 , wherein spherical nanoparticle assembled chains have a length of 400-500 nm.
15. The method of synthesizing the functionalized gold nanomaterial of claim 4 , wherein the molar concentration of the chloroauric acid aqueous solution added in step (1) is 1-6 mmol/L.
16. The method of synthesizing the functionalized gold nanomaterial of claim 4 , wherein the molar concentration of said N-(4-aminobutyl)-N-ethylisoluminol aqueous solution is 0.5-4.0 mmol/L.
17. The method of synthesizing the functionalized gold nanomaterial of claim 4 , wherein the molar concentration of the chloroauric acid aqueous solution added in step (2) is 1-6 mmol/L.
18. The method of synthesizing the functionalized gold nanomaterial as in claim 9 , wherein the temperature in the method step (1) and step (2) is in a range of 15-25° C.