Exchangeable alignment marker unit
An alignment marker unit for an object holder for microscopic imaging of an object with a microscope, wherein the alignment marker unit can be removed from and remounted on the object holder, wherein the alignment marker unit has at least one alignment marker for microscope-supported detection, and wherein the alignment marker is designed to determine a coordinate system for the object holder which allows for the microscope-supported calibration of the object or an area of the object relative to its position to the object holder and the position of the object holder relative to the microscope. Furthermore, an object holder system including an object holder and an alignment marker unit as described above is provided.
1. An alignment marker unit for an object holder for microscopic imaging of an object, wherein
the alignment marker unit can be removed from and remounted on the object holder and wherein the object holder supports the object and the object is removable and remountable on the object holder and wherein the object supports a sample to be microscopically imaged,
wherein the alignment marker unit comprises at least one alignment marker structured for microscope-supported detection, and
wherein the alignment marker is located and structured to determine a position and/or orientation of the object or an area of the object relative to the object holder.
2. The alignment marker unit according to claim 1 , wherein the alignment marker unit comprises first contact surfaces, which precisely fit corresponding second contact surfaces of the object holder.
3. The alignment marker unit according to claim 2 , wherein the first contact surfaces of the alignment marker unit are formed such that the alignment marker unit can be remounted azimuth-correctly on the object holder.
4. The alignment marker unit according to claim 1 , wherein the alignment marker unit has a two-piece design comprising at least one first contact element and a headpiece, and wherein the alignment marker is included in the headpiece.
5. The alignment marker unit according to claim 4 , wherein an outer surface of the first contact element comprises at least two differently designed partial surfaces.
6. The alignment marker unit according claim 4 , further comprising an additional contact element wherein the first contact element of the alignment marker unit can be extended by coupling the additional contact element to the first contact element.
7. The alignment marker unit according to claim 1 , wherein the alignment marker is L-shaped.
8. The alignment marker unit according to claim 1 , wherein the alignment marker comprises a double structure, and wherein units of the double structure are adapted to two different microscopy zoom levels.
9. The alignment marker unit according to claim 1 , wherein the alignment marker unit comprises at least three alignment markers.
10. The alignment marker unit according to claim 1 , wherein all elements of the alignment marker unit are electrically conducting and connected with one another in an electrically conducting manner.
11. An object holder system for microscopic imaging of an object, wherein the object holder system has at least a two-piece design comprising an object holder and at least one removable and remountable alignment marker unit according to claim 2 .
12. The object holder system according to claim 11 , wherein the first contact surfaces of the alignment marker unit have a clearance fit of less than 50 μm with the second contact surfaces of the object holder.
13. The object holder system according to claim 11 , further comprising a clamp connection or a screw connection whereby alignment marker unit is remounted on the object holder or wherein the alignment marker unit is remounted on the object holder by clamping.
14. The object holder system according to claim 11 , wherein the object holder and the alignment marker unit are height adjustable relative to one another.
15. The object holder system according to claim 11 , wherein the object holder system comprises at least three alignment marker units, which are independently remountable at a correct distance and a correct azimuth.
16. The object holder system according to claim 11 , wherein the object holder and the alignment marker unit are connected with one another in an electrically conducting manner when remounted.