IP Library Granted Patent US 9,089,151
Granted Patent B2
US 9,089,151 · App. 13/594,594 · Granted Jul 28, 2015

Enzyme producing

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Quick Facts
Patent No.
US 9,089,151
App. No.
13/594,594
Granted
Jul 28, 2015
Kind
B2
Abstract

The disclosure relates to enzyme producing Bacillus strains that provide benefits to animals and methods of using these strains. In one embodiment, the disclosure relates compositions comprising the enzyme producing Bacillus strains. In yet another embodiment, the disclosure relates to a feed for an animal comprising enzyme producing Bacillus strains.

Claims (17)

1. A biologically pure Bacillus strain in the form of a pellet having enzymatic activity selected from the group consisting of: Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus subtilis AGTP BS1069 (NRRL B-50544), Bacillus subtilis AGTP 944 (NRRL B-50548), Bacillus pumilus AGTP BS1068 (NRRL B-50543), and Bacillus pumilus KX11-1 (NRRL B-50546).

2. The strain of claim 1 , wherein the enzymatic activity is selected from the group consisting of cellulase activity, α-amylase activity, xylanase activity, esterase, β-mannanase, lipase activity, protease activity, and combinations thereof.

3. The strain of claim 1 , wherein the enzymatic activity is selected from the group consisting of zeinase activity and soy protease activity, and combinations thereof.

4. The strain of claim 1 , wherein, when the strain is administered to an animal, the strain provides an improvement in at least one of the following: body weight, average daily gain, average daily feed intake, feed efficiency, carcass characteristics, nutrient digestibility and manure waste problems.

5. The strain of claim 1 , wherein, when the strain is administered to an animal, the strain provides an improvement in at least one of the following: body weight, average daily gain, average daily feed intake, feed efficiency, carcass characteristics, nutrient digestibility and manure waste problems by at least 2% compared to a control.

6. The strain of claim 4 , wherein the animal is poultry or pig.

7. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS3BP5 (NRRL B-50510).

8. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS442 (NRRL B-50542).

9. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS521 (NRRL B-50545).

10. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS918 (NRRL B-50508).

11. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS1013 (NRRL B-50509).

12. The strain of claim 1 , wherein the Bacillus strain is Bacillus pumilus AGTP BS1068 (NRRL B-50543).

13. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis AGTP BS1069 (NRRL B-50544).

14. The strain of claim 1 , wherein the Bacillus strain is Bacillus subtilis B AGTP 944 (NRRL B-50548).

15. The strain of claim 1 , wherein the Bacillus strain is Bacillus pumilus KX11-1 (NRRL B-50546).

16. A biologically pure freeze-dried Bacillus strain selected from the group consisting of: Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP 1BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus subtilis AGTP BS1069 (NRRL B-50544), Bacillus subtilis AGTP 944 (NRRL B-50548), Bacillus pumilus AGTP BS1068 (NRRL B-50543), and Bacillus pumilus KX11-1 (NRRL B-50546).

17. A composition comprising a growth medium and an enzyme producing bacterial strain in the form of a pellet, wherein the bacterial strain is selected from the group consisting of: Bacillus subtilis AGTP BS3BP5 (NRRL B-50510), Bacillus subtilis AGTP BS442 (NRRL B-50542), Bacillus subtilis AGTP BS521 (NRRL B-50545), Bacillus subtilis AGTP BS918 (NRRL B-50508), Bacillus subtilis AGTP BS1013 (NRRL B-50509), Bacillus subtilis AGTP BS1069 (NRRL B-50544), Bacillus subtilis AGTP 944 (NRRL B-50548), Bacillus pumilus AGTP BS1068 (NRRL B-50543), and Bacillus pumilus KX11-1 (NRRL B-50546).

Assignments (2)
CHANGE OF NAME Recorded Feb 6, 2024
From: DUPONT NUTRITION BIOSCIENCES APS
To: INTERNATIONAL N&H DENMARK APS
Reel/Frame 066494/0814 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 14, 2012
From: DAVIS, MARI ELLEN; SAWALL, JUSTIN; NEUMANN, ANTHONY; SIRAGUSA, GREG; ROMERO, LUIS
To: DUPONT NUTRITION BIOSCIENCES APS
Reel/Frame 029298/0248 →