Imaging infection with compounds that bind to thymidine kinase
The instant invention provides a method for diagnosing an infection in a subject by administering to the subject a compound suitable for imaging which binds to a thymidine kinase present in the infecting organism, and obtaining an image of the subject to determine the presence and location of the compound, wherein a localization of the compound is indicative that the subject has an infection.
1. A method for monitoring the efficacy of treatment in a subject having a bacterial infection comprising: administering to the subject a first dose of a compound suitable for imaging which binds to a bacterial thymidine kinase present in the infecting organism; obtaining a first image of the subject to determine the presence and location of the compound; administering to the subject a second dose of a compound suitable for imaging which binds to a bacterial thymidine kinase present in the infecting organism at a time subsequent to the first administration; and obtaining a second image of the subject to determine the presence and location of the compound wherein the compound is a radiolabeled nucleoside; wherein a decrease in amount of compound localized to a particular location is indicative of effective therapy.
2. The method of claim 1 , wherein the image of the subject is acquired by a method selected from the group consisting of planar gamma imaging, single photon emission computed tomography (SPECT) and positron emission tomography (PET).
3. The method of claim 1 , wherein the compound is a radiolabeled nucleoside analog.
4. The method of claim 3 , wherein the nucleoside analog is labeled with radioactive fluorine or iodine.
5. The method of claim 4 , wherein the compound is selected from the group consisting of 2′-fluoro-2′deoxy-1-beta-D-arabinofuranosyl-5-iodo-uracil ([1251]-FIAU), 2′-fluoro-2′deoxy-1-beta-D-arabinofuranosyl-5-iodo-uracil ([1241]-FIAU), 9-(4-18F-fluoro-3-[hydroxymethyl]butyl)guanine ([18F]-FHBG), (18)F-1-(2′-deoxy-2′-fluoro-beta-d-arabinofuranosyl)thymine ([18F]-FMAU), 18F-2′-fluoro-2′deoxy-1beta-D-arabinofuranosyl-5-ethyl-uracil ([18F]-FEAU) and 1-(2′-deoxy-2′-fluoro-beta-D-arabinofuranosyl)-5-[18F]iodouracil ([18F]-FIAU).
6. The method of claim 3 , wherein the nucleoside analog emits gamma particles.
7. The method of claim 3 , wherein the nucleoside analog is fluorescent.
8. The method of claim 1 , wherein the bacterial infection is caused by bacteria from a genus selected from the group consisting of Escherichia, Bacillus, Chromobacterium, Clostridium, Enteroccus, Haemophilus, Listeria, Mycoplasma, Pasteruella, Salmonella, Staphylococcus, Streptococcus, Streptomyces, Vibrio , and Yersinia.