Transgenic soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity
This disclosure provides for transgenic soybean plants expressing a soybean homolog of glycine-rich protein 7 (GRP7) and exhibiting improved innate immunity and methods of making such plants.
1. A method of increasing the innate immunity exhibited by a soybean plant comprising:
introducing a transgene into a plurality of soybean cells to produce transgenic soybean cells, wherein the transgene comprises a nucleic acid sequence having at least 99% sequence identity to the nucleotide sequence of SEQ ID NO: 33 encoding soybean glycine rich RNA binding protein as set forth in SEQ ID NO: 34, wherein the nucleic acid sequence is operably linked to a promoter functional in soybean cells;
regenerating transgenic soybean plants from the transgenic soybean cells;
identifying at least one of said transgenic soybean plants expressing said transgene encoding said soybean glycine rich RNA binding protein;
exposing at least one of said transgenic soybean plants expressing said transgene encoding said soybean glycine rich RNA binding protein to a biotic stress; and
identifying at least one of said transgenic soybean plants that exhibits increased innate immunity in response to the biotic stress relative to a control plant.
2. The method of claim 1 , wherein said transgene comprises the nucleic acid sequence of SEQ ID NO: 33.
3. The method of claim 1 , wherein said biotic stress is selected from the group consisting of a pathogen-induced stress and a wound-induced stress.
4. The method of claim 1 , wherein the promoter is a constitutive promoter.
5. A method of increasing the innate immunity exhibited by a soybean plant comprising:
introducing a transgene into a plurality of soybean cells to produce transgenic soybean cells, wherein the transgene comprises a nucleic acid sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO: 34, wherein the nucleic acid sequence is operably linked to a promoter functional in soybean cells;
regenerating transgenic soybean plants from the transgenic soybean cells;
identifying at least one of said transgenic soybean plants expressing said transgene encoding said polypeptide;
exposing at least one of said transgenic soybean plants expressing said transgene to a biotic stress; and
identifying, at least one of said transgenic soybean plants that exhibits increased innate immunity in response to the biotic stress relative to a control plant.
6. The method of claim 5 , wherein said biotic stress is selected from the group consisting of a pathogen-induced stress and a wound-induced stress.
7. The method of claim 5 , wherein the promoter is a constitutive promoter.