Biomimetic hydroxyapatite synthesis
View Patent ↗A method for preparing nanoscale hydroxyapatite particles by combining an amount of a calcium ion source, which includes calcium acetate, and an amount of a phosphate ion source, wherein the amounts are sufficient to produce nanoscale hydroxyapatite particles and the amounts are combined under ambient conditions to produce the hydroxyapatite particles. Nanoscale hydroxyapatite particles are also presented.
1. A method for preparing nanoscale hydroxyapatite particles comprising combining an amount of a calcium ion source comprising calcium acetate and an amount of a phosphate ion source, wherein said amounts are sufficient to produce nanoscale hydroxyapatite particles consisting essentially of crystals characterized by a dominant lattice spacing of about 0.28 nm and said amounts are combined at a temperature between −10° C. and 45° C. to produce said hydroxyapatite particles by direct crystallization.
2. The method of claim 1 , wherein said phosphate ion source is selected from the group consisting of potassium orthophosphate, sodium orthophosphate, orthophosphoric acid, Group I phosphates, magnesium phosphate, ammonium phosphate, and a combination of two or more thereof.
3. The method of claim 2 , wherein said Group I phosphates are selected from the group consisting of monobasic sodium phosphate, dibasic sodium phosphate, tribasic sodium phosphate, monobasic potassium phosphate, dibasic potassium phosphate, and tribasic potassium phosphate.
4. The method of claim 1 , wherein said calcium ion source further comprises calcium hydroxide, calcium oxalate, calcium nitrate, calcium phosphate, calcium carbonate, calcium fluoride, calcium chloride, or a combination of two or more thereof.
5. The method of claim 1 further comprising agitating the combination until hydroxyapatite is formed.
6. The method of claim 1 , further comprising decreasing the temperature of the combined ion sources to a temperature not less than −10° C. until hydroxyapatite is formed.
7. The method of claim 1 , further comprising increasing the temperature of the combined ion sources to a temperature not greater than 45° C. until hydroxyapatite is formed.
8. The method of claim 1 , further comprising adding a buffer solution to the combination.
9. The method of claim 1 , wherein the production of said hydroxyapatite particles is stopped before the hexagonal structure is completely formed.