Polypeptides having transgalactosylating activity
View Patent ↗The present invention relates to polypeptides, specifically polypeptides having transgalactosylating activity and nucleic acids encoding these, and their uses in e.g. dairy product.
1. A method for producing a food product, the method comprising:
providing a substrate comprising lactose at a concentration of 5% w/w or greater;
adding a polypeptide to the substrate comprising lactose, the polypeptide having transgalactosylating activity, and the polypeptide selected from the group consisting of:
a. a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1,
b. a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2,
c. a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C. with,
i) the nucleic acid sequence comprised in SEQ ID NO: 10 encoding SEQ ID NO: 1,
ii) the cDNA sequence of i), or
iii) the complementary strand of i) or ii),
d. a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C. with,
i) the nucleic acid sequence comprised in SEQ ID NO: 11 encoding SEQ ID NO: 2,
ii) the cDNA sequence of i), or
iii) the complementary strand of i) or ii),
e. a polypeptide comprising a conservative substitution, deletion or insertion of in a sequence resulting in a sequence having at least 90% identity to SEQ ID NO:1, or
f. a polypeptide comprising a conservative substitution, deletion or insertion of amino acids in a sequence resulting in a sequence having at least 90% identity to SEQ ID NO: 2,
provided that the polypeptide of above items a, c, and e, at the most has a length of 1806 amino acids and provided that the polypeptide of above items b, d, and f at the most has a length of 1767 amino acids; and
producing the food product that contains galacto-oligossacharides.
2. A method of producing a dairy product, the method comprising:
providing a milk-based substrate comprising lactose at a concentration of 5% w/w or greater;
adding a polypeptide to the substrate comprising lactose, the polypeptide having transgalactosylating activity, and the polypeptide selected from the group consisting of:
a. a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 1,
b. a polypeptide comprising an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2,
c. a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C. with,
i) the nucleic acid sequence comprised in SEQ ID NO: 10 encoding SEQ ID NO: 1,
ii) the cDNA sequence of i), or
iii) the complementary strand of i) or ii),
d. a polypeptide encoded by a polynucleotide that hybridizes under high stringency conditions of 0.1 SSC (0.15 M NaCl, pH 7.0) at 65° C. with,
i) the nucleic acid sequence comprised in SEQ ID NO: 11 encoding SEQ ID NO: 2,
ii) the cDNA sequence of i), or
iii) the complementary strand of i) or ii),
e. a polypeptide comprising a conservative substitution, deletion or insertion of in a sequence resulting in a sequence having at least 90% identity to SEQ ID NO:1, or
f. a polypeptide comprising a conservative substitution, deletion or insertion of amino acids in a sequence resulting in a sequence having at least 90% identity to SEQ ID NO: 2,
provided that the polypeptide of above items a, c, and e, at the most has a length of 1806 amino acids and provided that the polypeptide of above items b, d, and f at the most has a length of 1767 amino acids; and
producing the dairy product that contains galacto-oligossacharides.
3. The method of claim 1 , further comprising treating the substrate with a hydrolysing beta-galactosidase.