Staining composition
The invention is directed to a staining composition and to the use of the staining composition in staining ocular tissue. In a first aspect, the invention provides a staining composition comprising a vital dye and a density increasing compound chosen from the group consisting of water soluble polymers and small inert molecules.
1. A method of treatment comprising,
staining an ocular tissue or part of an ocular tissue with a staining composition,
wherein the staining composition comprises a first and a second dye,
wherein the first dye is trypan blue (TB) and the second dye is brilliant blue G (BBG), and
wherein a concentration of polyethylene glycol in the staining composition is 2-10 wt. %.
2. The method according to claim 1 , wherein the staining composition further comprises a density increasing compound and wherein the density increasing compound is an iodinated organic compound.
3. The method according to claim 1 , wherein the polyethylene glycol has a molecular weight of 50-500,000 g/mol.
4. The method according to claim 1 , wherein the weight ratio of the first dye to the second dye in the dye of the staining composition is between 1:1 and 100:1.
5. The method according to claim 1 , wherein the staining composition further comprises a salt, wherein the salt is sodium chloride, potassium chloride, calcium chloride, magnesium chloride, or a combination thereof.
6. The method according to claim 1 , wherein the staining composition is an aqueous solution, a colloidal dispersion or a viscous or viscoelastomeric solution, and wherein the concentration of the in the staining composition is 2-10 wt. %.
7. The method according to claim 4 , wherein the staining composition has a density at 298 K of more than 1003 and less than 1040 kg*m −3 .
8. The method according to claim 4 , wherein the staining composition has a viscosity of at least 2.0 mPa·s, as determined using a rheometer at 298 K.
9. The method according to claim 1 , wherein the concentration of the first dye in the staining composition is 0.001-2 wt. %.
10. A method for preparing a staining composition, comprising the step of dissolving a first dye and a second dye,
wherein the first dye is trypan blue (TB) and the second dye is brilliant blue G (BBG), and
polyethylene glycol in a liquid; and dissolving a salt in the liquid to adjust the osmolarity of the liquid to a value between 250 and 400 mosmol/L.
11. The method according to claim 1 , wherein the treatment is part of an eye surgery.
12. The method according to claim 2 , wherein the density increasing compound is an iodixanol.
13. The method according to claim 2 , wherein the density increasing compound is 5-N(N-2,3-dihydroxypropylacetamido)-2,4,6-tri-iodo-N,N′-bis(2,3-dihydroxypropyl) isophtalamide.
14. The method according to claim 1 , wherein the polyethylene glycol has a molecular weight of 300-100,000 g/mol.
15. The method according to claim 1 , wherein the polyethylene glycol has a molecular weight of 600-40,000 g/mol.
16. The method according to claim 1 , wherein the weight ratio of the first dye to the second dye in the dye of the staining composition is between 2:1 and 25:1.
17. The method according to claim 1 , wherein the weight ratio of the first dye to the second dye in the dye of the staining composition is between 4:1 and 15:1.
18. The method according to claim 1 , wherein the staining composition is an aqueous solution, a colloidal dispersion or a viscous or viscoelastomeric solution, wherein the concentration of the polyethylene glycol in the staining composition is 3-6 wt. %.
19. The method according to claim 4 , wherein the staining composition has a density at 298 K of more than 1004-1026 kg·m −3 .
20. The method according to claim 4 , wherein the staining composition has a viscosity of at least 2.2 mPa·s, as determined using a rheometer at 298 K.
21. The method according to claim 4 , wherein the staining composition has a viscosity of at least 2.5 mPa·s, as determined using a rheometer at 298 K.
22. The method according to claim 1 , wherein the concentration of the first dye in the staining composition is 0.01-1 wt. %.
23. The method according to claim 1 , wherein the concentration of the first dye in the staining composition is 0.1-0.5 wt. %.
24. The method according to claim 1 , wherein the treatment is part of a vitreo-retinal surgery.
25. The method according to claim 1 , wherein the treatment is part of a capsulorhexis procedure.