Conditioned medium obtained from stem cells and its use in therapy
Described herein are conditioned medium and processed conditioned medium, each of which comprises secreted stem cell factors; compositions containing conditioned medium and/or processed conditioned medium and a delivery polymer. The conditioned medium, processed conditioned medium and compositions may be used to promote blood vessel growth and healing of injured tissues.
1. A method of producing processed conditioned medium, comprising:
(a) culturing stem cells in growth-factor-free medium comprising desferroxamine, wherein the stem cells have been previously cultured in growth medium, thereby generating conditioned medium that comprises factors secreted by the stem cells;
(b) harvesting the conditioned medium; and
(c) filtering the conditioned medium to produce processed conditioned medium.
2. The method of claim 1 , wherein the filtering is by ultra-filtration.
3. The method of claim 1 , wherein the processed conditioned medium comprises an increased concentration of angiogenic factors.
4. The method of claim 1 , wherein the stem cells are isolated from bone marrow or adipose tissue.
5. The method of claim 4 , wherein the stem cells are isolated from bone marrow and are mesenchymal stem cells.
6. The method of claim 4 , wherein the stem cells are isolated from bone marrow and are hematopoietic stem cells.
7. A method of enhancing secretion of angiogenic factors by stem cells, comprising culturing the stem cells in growth factor-free medium comprising desferroxamine.
8. The method of claim 7 , wherein the stem cells are isolated from bone marrow or adipose tissue.
9. The method of claim 8 , wherein the stem cells are isolated from bone marrow and are mesenchymal stem cells.
10. The method of claim 8 , wherein the stem cells are isolated from bone marrow and are hematopoietic stem cells.