Immunoassay for chromogranin A, antibodies and kit
The invention relates to monoclonal antibodies which are reactive with an epitope in the polypeptide represented by amino acid sequence 236 to 251 or 264 to 279 of the human CGA amino acid sequence. The invention further relates to the use of these monoclonal antibodies in an immunoassay for CGA, to immunoreagents comprising any of these two antibodies, and to test kits for the determination of CGA containing immunoreagents based on both of the monoclonal antibodies.
1. A method for the determination of a human Chromogranin A (CGA) polypeptide or fragments thereof comprising SEQ ID NO:2 and SEQ ID NO:3, comprising the steps of:
reacting a sample with a set of monoclonal antibodies, wherein at least one monoclonal antibody in the set is reactive with an epitope within amino acids 236 to 251 of human CGA (SEQ ID NO:2); and wherein at least one other monoclonal antibody in the set is reactive with an epitope within amino acids 264 to 279 of human CGA (SEQ ID NO:3), and
detecting binding of the set of monoclonal antibodies, thereby detecting human CGA polypeptide of fragments thereof comprising SEQ ID NO:2 and SEQ ID NO:3 in the sample.