METHODS FOR EXTRACTING PLATELETS AND COMPOSITIONS OBTAINED THEREFROM
This invention provides methods for extracting platelets, compositions obtained therefore, and methods for using the same.
1 . A composition comprising a growth factor produced by a process comprising:
disrupting pelletized platelets in an extraction solution to produce an insoluble material portion and the extraction solution comprising a growth factor; and
removing insoluble materials and proteins having molecular weight of 2 kD or less from said extraction solution to produce a composition comprising a growth factor.
2 . The composition of claim 1 , wherein said method for disrupting pelletized platelets comprises sonication, homogenization, nitrogen cavitation, a French pressure cell, or a combination thereof.
3 . The composition of claim 1 , wherein said step of removing insoluble materials and proteins comprises:
(i) filtering said extraction solution using an ultrafiltration process to remove insoluble materials and proteins having molecular weight of 2 kD or less; or
(ii) removing insoluble materials from said extraction solution; and
(a) dialyzing said extraction solution against an acidic solution using a semipermeable membrane having 2 kD or less MWCO; or
(b) filtering said extraction solution using an ultrafiltration process to remove proteins having molecular weight of 2 kD or less.
4 . The composition of claim 3 , wherein said step of dialyzing comprises using a semipermeable membrane having 8 kD or less MWCO.
5 . The composition of claim 1 , wherein said process further comprises subjecting said extraction solution to an ultrafiltration process to remove any microorganisms that maybe present.
6 . The composition of claim 5 , wherein said process further comprises lyophilizing said extraction solution to produce a solid composition comprising the growth factor.
7 . The composition of claim 6 , wherein said process further comprises dissolving said solid composition in a solution to produce a fluid composition comprising the growth factor.
8 . The composition of claim 1 , wherein said growth factor comprises VEGF, TGF-β 1 , TGF-β 2 , PDGF-BB, EGF, or a mixture thereof.
9 . A wound healing composition comprising therapeutically effective amount of a composition of claim 1 and a pharmaceutically acceptable carrier.
10 . The wound healing composition of claim 9 , wherein said pharmaceutically acceptable carrier comprises a bandage.
11 . The wound healing composition of claim 9 , wherein the pharmaceutically acceptable carrier comprises a pharmaceutically acceptable polymer.
12 . A method for regenerating a tissue or treating a wound comprising contacting a desired site for tissue regeneration or the wound with an angiogenic composition comprising a composition of claim 1 .
13 . The method of claim 12 , wherein the desired site for tissue regeneration comprises a burn site, a graft donor site, an oral and maxillofacial surgery site, a mucogingival surgery site, a perioplastic surgery site, an oral mucosa site, or a combination thereof.
14 . A method for reducing the risk of microbial infection in a wound comprising treating the wound with a wound treatment composition comprising a composition of claim 1 .