Methods and compositions to protect aquatic invertebrates from disease
Compositions and methods of protecting aquatic invertebrates from disease is shown. In one embodiment, dsRNA or antisense RNA to a nucleic acid molecule of the disease-causing microorganism is prepared and delivered to the animal. In another embodiment, a nucleic acid molecule of the disease-causing microorganism is delivered to the animal. In another embodiment, the RNA or nucleic acid molecule is delivered to the animal by replicon particle. In a further embodiment, the protective molecule is delivered to the digestive tract of the animal. Protection from disease is obtained.
1. A method of protecting shrimp from Infectious Myonecrosis Virus (IMNV), the method comprising,
a) identifying a target nucleic acid molecule of IMNV;
b) preparing a composition comprising a protective molecule comprising
(i) a dsRNA molecule comprising at least one RNA molecule that is capable of hybridizing to said target nucleic acid molecule of said IMNV;
wherein said composition does not comprise an expression cassette or vector comprising said protective molecule; and
c) administering said composition comprising said protective molecule to said shrimp wherein said shrimp is protected from IMNV.
2. The method of claim 1 , wherein said composition comprising said protective molecule is administered by immersing said shrimp in a solution comprising said composition comprising said protective molecule.
3. The method of claim 1 , wherein said composition comprising said protective molecule is administered by oral administration.
4. The method of claim 1 , further comprising immersing said shrimp in a solution comprising said composition comprising said protective molecule, and orally administering said composition comprising said protective molecule to said shrimp.
5. The method of claim 1 , wherein said shrimp is protected from said IMNV for a period of time selected from the group consisting of at least 20 days, at least 25 days, at least 30 days, at least 40 days and at least 60 days following administration of said protective molecule.
6. The method of claim 1 , wherein said dsRNA is produced by a nucleic acid molecule selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 51, SEQ ID NO: 56, and SEQ ID NO: 61 or the complement thereof, and a fragment of said nucleic acid molecule wherein said dsRNA produces a protective response.
7. The method of claim 1 , wherein said dsRNA comprises a sequence that corresponds to all or a portion of said target nucleic acid molecule and a sequence that is the full or partial complement of said target nucleic acid molecule, wherein said sequence that corresponds to all or a portion of said target nucleic acid molecule is selected from the group consisting of SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 52, SEQ ID NO: 57, and SEQ ID NO: 62 and a fragment thereof, and said RNA that is the full or partial complement of all or a portion of said target nucleic acid molecule is selected from the group consisting of SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 53, SEQ ID NO: 58, and SEQ ID NO: 63 and a fragment thereof.
8. The method of claim 1 , wherein said target molecule comprises a nucleotide sequence encoding SEQ ID NO: 68 or fragment thereof wherein said protective molecule when administered to said shrimp, protects said shrimp from IMNV.
9. The method of claim 1 , wherein said target molecule comprises SEQ ID NO: 72 or fragment thereof wherein said protective molecule when administered to said shrimp, protects said shrimp from IMNV.
10. A method of protecting shrimp from Infectious Myonecrosis Virus (IMNV), the method comprising orally administering to said shrimp a composition comprising a protective molecule, said protective molecule comprising a dsRNA, said dsRNA capable of interfering with said target nucleic acid molecule of said IMNV, wherein said composition does not comprise an expression cassette or vector comprising said protective molecule and wherein said shrimp is protected from said IMNV.
11. The method of claim 9 , wherein said shrimp is protected from said IMNV for a period of time selected from the group consisting of at least 20 days, at least 25 days, at least 30 days, at least 40 days and at least 60 days following administration of said protective molecule.
12. The method of claim 9 , wherein said dsRNA is produced by a nucleic acid molecule selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 51, SEQ ID NO: 56, and SEQ ID NO: 61 or the complement thereof, and a fragment of said nucleic acid molecule thereof wherein said dsRNA produces a protective response.
13. The method of claim 9 , wherein said dsRNA comprises an sequence that corresponds to all or a portion of said target nucleic acid molecule and a sequence that is the full or partial complement of said target nucleic acid molecule, wherein said sequence that corresponds to all or a portion of said target nucleic acid molecule is selected from the group consisting of SEQ ID NO: 35, SEQ ID NO: 37, SEQ ID NO: 39, SEQ ID NO: 52, SEQ ID NO: 57, and SEQ ID NO: 62 and a fragment thereof, and said sequence that is the full or partially complement to all or a portion of said target nucleic acid molecule is selected from the group consisting of SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, \SEQ ID NO: 53, SEQ ID NO: 58, and SEQ ID NO: 63 and a fragment thereof.
14. The method of claim 9 , wherein said target molecule comprises a nucleotide sequence encoding SEQ ID NO: 68 or fragment thereof wherein said fragment produces a protective molecule that when administered to said shrimp, protects said shrimp from IMNV.
15. The method of claim 9 , wherein said target molecule comprises SEQ ID NO: 72 or fragment thereof wherein said fragment produces a protective molecule that when administered to said shrimp, protects said shrimp from IMNV.