Microscope with tunable acoustic gradient index of refraction lens enabling multiple focal plan imaging

1. A microscope, comprising:

a stage configured to receive an item;

a tunable acoustic gradient index of refraction lens having a first aspect positioned to image the received item, wherein the first aspect of the lens is configured to have an optical power profile in accordance with an operational frequency of the tunable acoustic gradient index of refraction lens;

one or more lenses configured to magnify an image of the received item at a viewing point;

at least one pulsed light source configured to illuminate the received item and to pulse at one or more points within the optical power profile of the tunable acoustic gradient index of refraction lens; and

an eyepiece configured to provide the viewing point of the magnified, illuminated item image.

2. The microscope of claim 1 , wherein the tunable acoustic gradient index of refraction lens images as a single stage lens.

3. The microscope of claim 1 , wherein the tunable acoustic gradient index of refraction lens images as a multi-stage lens.

4. The microscope of claim 1 , wherein the one or more lenses configured to magnify the image comprise at least a second aspect of the tunable acoustic gradient index of refraction lens.

5. The microscope of claim 1 , further comprising a condenser lens configured to focus the illumination.

6. The microscope of claim 1 , wherein the pulsed light source comprises a coherent source.

7. The microscope of claim 1 , further comprising a synchronizer configured to synchronize the imaging by the tunable acoustic gradient index of refraction lens to the illumination provided by a light source.

8. The microscope of claim 7 , wherein the synchronizer comprises at least a tunable acoustic gradient index of refraction lens driving signal.

9. The microscope of claim 8 , wherein the driving signal is at about 143 kHz.

10. The microscope of claim 1 , wherein the illumination pulse provided by the pulsed light source provides a high depth of field.

11. The microscope of claim 1 , wherein the pulsed light source comprises a white light emitting diode.

12. The microscope of claim 1 , wherein the pulses are of length 300 ns.

13. A microscope, comprising:

a stage configured to receive an item;

a tunable acoustic gradient index of refraction lens positioned to image the received item, wherein the tunable acoustic gradient index of refraction lens is configured to have an optical power profile in accordance with an operational frequency of the tunable acoustic gradient index of refraction lens;

one or more lenses configured to magnify an image of the received item at a viewing point;

a first pulsed light source having a first color and configured to illuminate the received item and to pulse at a first point within the optical power profile of the tunable acoustic gradient index of refraction lens; and

a second pulsed light source having a second color and configured to illuminate the received item and to pulse at a second point within the optical power profile of the tunable acoustic gradient index of refraction lens, wherein the first point is different from the second point.

14. The microscope of claim 13 , wherein the first pulsed light source comprises a light emitting diode having the first color of red.

15. The microscope of claim 13 , wherein the second pulsed light source comprises a light emitting diode having the second color of blue.

16. The microscope of claim 13 , wherein the tunable acoustic gradient index of refraction lens images as a single stage lens.

17. The microscope of claim 13 , wherein the tunable acoustic gradient index of refraction lens images as a multi-stage lens.

18. A method of microscopy, comprising:

configuring a stage to receive an item;

positioning a tunable acoustic gradient index of refraction lens to image the received item, and to have an optical power profile in accordance with an operational frequency of the tunable acoustic gradient index of refraction lens;

configuring one or more lenses to magnify an image of the received item at a viewing point;

pulsing a first light source having a first color to illuminate the received item and to pulse at a first point within the optical power profile of the tunable acoustic gradient index of refraction lens; and

pulsing a second light source having a second color to illuminate the received item and to pulse at a second point within the optical power profile of the tunable acoustic gradient index of refraction lens, wherein the first point is different from the second point.