Device to expose cells to fluid shear forces and associated systems and methods
View Patent ↗The present technology relates generally to devices to expose cells to fluid shear forces and associated systems and methods. In particular, several embodiments are directed toward devices to expose cells to fluid shear forces in order to measure changes in internal cell forces. In some embodiments, a fluidic device includes a flow unit configured to induce fluid flow through the device. The device further includes a fluid channel configured to accept a biological sample dispersed on an array of flexible structures. The flow unit can be configured to induce disturbed and/or laminar flow in the fluid channel. The device can further include optical or magnetic detection means configured to measure a degree of deflection of one or more flexible structures in the array.
1. An analytic method, comprising:
placing a plurality of flexible microposts in a fluid flow chamber, wherein a plurality of cells is dispersed over at least two of the plurality of microposts;
flowing fluid over the microposts, wherein flowing fluid includes inducing laminar and disturbed flow to the plurality of cells; and
measuring a deflection of the microposts.
2. The method of claim 1 wherein inducing laminar and disturbed flow comprises flowing fluid over a backward-facing step in the fluid flow chamber, wherein the laminar and disturbed flow occur downstream of the step.
3. The method of claim 1 wherein placing the plurality of microposts includes placing microposts having human pulmonary artery endothelial cells bound thereto.
4. The method of claim 1 , further comprising placing an air chamber in the fluid flow chamber to induce a steady flow rate.
5. The method of claim 1 wherein flowing fluid over the microposts comprises flowing a fluid containing at least one of a drug, reagent, nucleotide, protein.
6. The method of claim 1 , further comprising determining an intercellular tension between at least two of the plurality of cells based on the measured deflection.
7. The method of claim 1 , wherein at least one of the plurality of cells is in simultaneous, direct contact with two of the microposts.
8. The method of claim 1 , further comprising seeding the plurality of cells over and across the top surfaces of the microposts.