Inhibition and dispersion of bacterial biofilms with 2-aminobenzimidazole derivatives
Compounds described herein inhibit biofilm formation or disperse pre-formed biofilms of Gram-negative bacteria. Biofilm-inhibitory compounds can be encapsulated or contained in a polymer matrix for controlled release. Coatings, films, multilayer films, hydrogels, microspheres and nanospheres as well as pharmaceutical compositions and disinfecting compositions containing biofilm-inhibitory compounds are also provided. Methods for inhibiting formation of biofilms or dispersing already formed biofilms are provided. Methods for treating infections of gram-negative bacteria which form biofilms, particularly those of Pseudomonas and more particularly P. aeruginosa.
1. A method for inhibiting the formation of a biofilm or dispersing an already-formed biofilm of Pseudomonas aeruginosa which comprises the step of contacting the Pseudomonas aeruginosa or the biofilm thereof with an effective amount of one or more compounds selected from: 5-iodo-2-aminobenzimidazole; 5-bromo-2-aminobenzimidazole, 5-chloro-2-aminobenzimidazole, 5-fluoro-2-aminobenzimidazole, 5-nitro-2-aminobenzimidazole, 5-methyl-2-aminobenzimidazole, 4-methyl-2-aminobenzimidazole, 5,6-dimethyl-2-aminobenzimidazole, 5-methoxy-2-aminobenzimidazole, and 2-amino-1H-naphtho[2,3-d]imidazole.
2. The method of claim 1 , wherein an already-formed biofilm of Pseudomonas aeruginosa is dispersed.
3. The method of claim 2 , wherein the one or more compound is 5,6-dimethyl-2-aminobenzimidazole.
4. The method of claim 1 , wherein the one or more compound is 5,6-dimethyl-2-aminobenzimidazole.
5. The method of claim 1 , wherein the one or more compounds are selected from: 5-iodo-2-aminobenzimidazole; 5-bromo-2-aminobenzimidazole, 5-chloro-2-aminobenzimidazole, 5-fluoro-2-aminobenzimidazole, and 5-nitro-2-aminobenzimidazole.
6. The method of claim 5 , wherein an already-formed biofilm of Pseudomonas aeruginosa is dispersed.
7. The method of claim 1 , wherein the one or more compounds are selected from: 5-methyl-2-aminobenzimidazole, 4-methyl-2-aminobenzimidazole, 5,6-dimethyl-2-aminobenzimidazole, and 5-methoxy-2-aminobenzimidazole.
8. The method of claim 7 , wherein an already-formed biofilm of Pseudomonas aeruginosa is dispersed.
9. The method of claim 1 , wherein contacting includes release of the one or more compounds from a film, multilayer film, coating, hydrogel, microsphere or nanosphere.