Detoxified pneumococcal neuraminidase and uses thereof
Provided herein are compositions designed to reduce or prevent bacterial infections (for example pneuomococcal infections), nasal carriage, nasal colonization, and central nervous system invasion. Provided herein is a composition comprising a polypeptide comprising the amino acid sequence of SEQ ID NO:19 or a variant thereof that can elicit an anti-neuraminidase immune response. Further provided are methods of making and using the compositions disclosed herein. Specifically provided are methods of generating antibodies in a subject comprising administering to the subject an agent or composition taught herein. Also provided are methods of reducing or preventing nasal carriage or pneumococcal infection in a subject comprising administering to the subject a composition taught herein.
1. A method of reducing pneumococcal nasal carriage in a subject comprising administering to the subject a polypeptide comprising the amino acid sequence of SEQ ID NO:19, a polypeptide comprising the amino acid sequence of SEQ ID NO:35, or a polypeptide comprising the amino acid sequence of SEQ ID NO:36, wherein the polypeptide elicits an anti-neuraminidase immune response in the subject, wherein the polypeptide is administered under conditions that reduce or prevent the nasal carriage, and wherein the polypeptide does not consist of SEQ ID NO:15 or SEQ ID NO:16.