Highly fluorescent polymer nanoparticle
View Patent ↗Fluorescent nanoparticles are provided. The nanoparticles are formed from poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylene-1,4-phenylene)]. Also provided are methods for imaging a target to which the nanoparticles are bound.
1. A nanoparticle consisting essentially of:
poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-(1-cyanovinylene-1,4-phenylene)];
a copolymer; and
a binding moiety covalently attached to the copolymer, wherein the binding moiety is a protein, and wherein the nanoparticle has a hydrodynamic diameter of 1-30 nm and a quantum yield of about 60%.
2. The nanoparticle of claim 1 , wherein the hydrodynamic diameter is 20 nm or less.
3. The nanoparticle of claim 1 , having a fluorescence emission peak in the range of about 590 nm to about 620 nm.
4. The nanoparticle of claim 1 , wherein the nanoparticle is formed using nanoprecipitation.
5. The nanoparticle of claim 1 , wherein the copolymer is poly(styrene-co-maleic anhydride).
6. The nanoparticle of claim 1 , wherein the binding moiety is positioned at the surface of the nanoparticle.