Methods of inhibiting growth of a neoplastic cell
Disclosed are methods of preparing and using placentally-derived stem cells and compositions useful for the treatment of cancer. Said stem cells and compositions function through inducing a “guided differentiation” program in cancer cells, thereby reducing malignancy. Further extension of the invention pertains to augmenting ability of administered cells to induce differentiation through the co-administration of known differentiation inducing agents. Within the context of this disclosure, methods for inducing host responses to cancer are also described.
1. A method of inhibiting growth of a neoplastic cell, the method comprising:
(a) providing a cell free-placental extract made by the process comprising:
(i) obtaining human placenta;
(ii) homogenizing the obtained human placenta by digesting the obtained human placenta with a collagenase and a heparitinase, wherein the heparitinase digestion is at a neutral pH, the homogenization resulting in a homogenate;
(iii) centrifuging said homogenate to produce a supernatant free of cellular debris; and
(iv) purifying the supernatant to obtain cell free-placental extract comprising a cell differentiation-inducing fraction having a molecular weight in the range from about 10 Daltons to about 10,000 Daltons
(b) directly administering said cell free-placental extract to the neoplastic cell which is selected from the group consisting of a leukemia cell, and a prostate cancer cell, thereby inhibiting growth of the neoplastic cell.
2. The method of claim 1 , wherein the step (iv) of purifying comprises running the supernatant through adsorption on a C-18 column, and then filter sterilizing the purified supernatant.
3. The method of claim 1 , wherein the neoplastic cell is in vivo.
4. The method of claim 1 , wherein the neoplastic cell is in vitro.
5. The method of claim 1 , wherein the administered cell free-placental extract is in a liposomal complex.
6. The method of claim 1 , wherein a histone deacetylase inhibitor is added to the extract prior to the administration of said cell free placenta.
7. The method of claim 1 , wherein the step(ii) further comprises sonicating the the homogenate.