Methods of treatment using ex vivo expansion of cord blood T cells
Provided are methods of enhancing ex vivo proliferation of a T cell population, the methods comprising contacting the T cell population with IL-7 and anti-CD3/CD28 antibody to activate and expand the T cell population. Further provided are methods of generating an antigen-specific cytotoxic T cell population comprising priming a CD3/CD28-expanded T cell population against an antigen (e.g., a cancer cell) in the presence of at least one of IL-7, IL-12, and IL-15, or a combination thereof. Further provided are methods of treating T cell lymphopenia in a subject, comprising administering a CD3/CD28-expanded T cell population to the subject.
1. A method of enhancing ex vivo proliferation and activation, relative to no stimulation, of a T cell population from cord blood, the method consisting of contacting the T cell population with IL-7, or a functional fragment thereof, IL-2, or a functional fragment thereof, an anti-CD3 antibody, or a functional fragment thereof, and an anti-CD28 antibody, or a functional fragments thereof, to activate and proliferate the T cell population.
2. The method of claim 1 , wherein the anti-CD3 antibody, or functional fragment thereof, or the anti-CD28 antibody, or functional fragment thereof, is linked to a surface.
3. The method of claim 1 , wherein the anti-CD3 antibody, or functional fragment thereof, and the anti-CD28 antibody, or functional fragment thereof, are linked to a surface.
4. The method of claim 3 , wherein the anti-CD3 antibody, or functional fragment thereof, and the anti-CD28 antibody, or functional fragment thereof, are linked to the same surface.
5. The method of claim 2 , wherein the surface comprises a cell culture vessel, a glass particle, a latex particle, a paramagnetic particle, a microparticle, or a nanoparticle.
6. The method of claim 1 , wherein the T cell population is induced to proliferate to about 100-fold the original T cell population.
7. The method of claim 1 wherein the expanded T cell population retains the capacity for in vivo proliferation upon transplantation.