IP Library › Patent Application 13701329
Patent Application
App. No. 13/701,329

NATIVE WHARTON'S JELLY STEM CELLS AND THEIR PURIFICATION

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Patent No.
US None
App. No.
13/701,329
Abstract

Noncultured Wharton's Jelly stem cells and methods of their purification, storage and use are provided.

Claims (28)

1 . A method of purifying noncultured Wharton's Jelly stem cells, the method comprising separating noncultured Wharton's Jelly stem cells from a digested tissue comprising Wharton's Jelly.

2 . The method of claim 1 , further comprising digesting a tissue comprising Wharton's Jelly.

3 - 5 . (canceled)

6 . A method according to claim 2 , wherein the tissue is digested by mechanically increasing the surface area of the tissue.

7 . The method of claim 6 , comprising cutting the tissue.

8 . The method of claim 6 , comprising mincing the tissue.

9 . (canceled)

10 . A method according to claim 2 , further comprising separating digested and undigested tissue.

11 . The method of claim 10 , comprising sedimenting the undigested tissue.

12 . The method of claim 11 , wherein centrifugation is used for sedimenting the undigested tissue.

13 . The method of claim 10 , comprising filtering the digested tissue.

14 . The method of claim 13 , wherein the noncultured Wharton's Jelly stem cells are separated from the filtrate.

15 . A method according to claim 10 , further comprising washing or diluting the digested tissue before separating it from the undigested tissue.

16 . A method of purifying cultured and noncultured Wharton's Jelly stem cells, the method comprising:

purifying noncultured Wharton's Jelly stem cells according to claim 10 ; and

culturing mesenchymal stem cells from the undigested tissue.

17 . The method of claim 16 , wherein the mesenchymal stem cells are cultured in digested Wharton's Jelly.

18 . Purified, noncultured Wharton's Jelly stem cells substantially free of semi-solid Wharton's Jelly.

19 . Purified, noncultured Wharton's Jelly stem cells at a temperature below −20° C.

20 . Wharton's Jelly stem cells according to claim 18 , wherein the cells have reduced CD73 and reduced CD105 levels compared to cultured mesenchymal stem cells.

21 . Wharton's Jelly stem cells according to claim 18 , wherein the Wharton's Jelly stem cells are maintained in a sterile solution.

22 . (canceled)

23 . A method of treating a subject, the method comprising administering to the subject Wharton's Jelly stem cells according to claim 18 .

24 . (canceled)

25 . A homogeneous solution comprising Wharton's Jelly.

26 - 28 . (canceled)

29 . A method of maintaining a cell comprising mixing the cell in the solution of claim 25 .

30 - 32 . (canceled)