Method for non-covalent immobilization of infectious prion protein
The present invention is method for non-covalently immobilizing an infectious prion protein using a magnetic substrate.
1. A method for immobilizing an infectious prion protein comprising contacting an infectious prion protein with a magnetic substrate that selectively immobilizes the infectious prion protein and not PrP c so that the infectious prion protein is immobilized.
2. The method of claim 1 , wherein the magnetic substrate comprises an iron oxide.
3. The method of claim 1 , wherein the magnetic substrate is silanized.
4. The method of claim 1 , wherein the magnetic substrate is a microparticle, nanoparticle, or nanopowder.
5. The method of claim 1 , wherein the infectious prion protein is present in a biological sample.
6. A complex comprising an infectious prion protein and a manufactured magnetic substrate, wherein the infectious prion protein is non-covalently bound to the substrate, in the absence of a cross-linking agent, so that the infectious prion protein is immobilized.