Methods for reducing the blood priming volume and membrane surface area in microfluidic lung assist devices
A device and method for oxygenating blood is disclosed herein. The device includes a plurality of passive mixing elements that causes a fluid to mix as it flows through the device. The passive mixing elements continually expose new red blood cells to the portion of the flow channel where oxygenation can occur. Accordingly, in some implementations, the device and method uses less blood to prime the device and allows for the oxygenation of blood with a substantial shorter flow channel when compared to conventional oxygenation methods and devices.
1. A microfluidic oxygenation device comprising:
a first polymer layer defining a first oxygen flow channel therein;
a second polymer layer defining a first blood flow channel therein, the first blood flow channel overlapping the first oxygen flow channel, and the first blood flow channel further comprising a plurality of passive mixing elements spaced sequentially along a length of a first wall of the first blood flow channel, wherein the plurality of passive mixing elements have a height or a depth less than about 30% of a height of the first blood flow channel and are configured to redistribute a fluid flowing through the first blood flow channel within the channel; and
a membrane separating the first oxygen flow channel and the first blood flow channel at the overlapping portions of the channels, the membrane allowing communication between the first oxygen flow channel and the first blood flow channel.
2. The device of claim 1 , wherein each of the plurality of passive mixing elements comprises one of a straight ridge, an angled ridge, a chevron canal, a dome, a cone, a pit or a post.
3. The device of claim 1 , wherein a first fluid flows through the first oxygen flow channel and a second fluid flows through the first blood flow channel.
4. The device of claim 3 , wherein the first fluid is oxygen and the second fluid is deoxygenated blood.
5. The device of claim 1 , wherein the first wall of the first blood flow channel is the floor of the first blood flow channel.
6. The device of claim 1 , wherein the height of the first blood flow channel is between about 10 and 100 microns.
7. The device of claim 1 , wherein the membrane thickness is between about 10 and about 50 microns.
8. The device of claim 1 , wherein the length of the first oxygen flow channel and the first blood flow channel is between about 1 mm and about 50 mm.
9. The device of claim 1 , where the width of the first blood flow channel is between about 100 microns and 200 microns.
10. The device of claim 1 , wherein the membrane is permeable to oxygen and carbon dioxide.
11. The device of claim 1 , wherein the walls of the first blood flow channel are coated with an anticoagulant.
12. The device of claim 1 , wherein the device includes a second blood flow channel separated from the first oxygen flow channel by a second permeable membrane.
13. A method for oxygenating deoxygenated blood, the method comprising:
providing a microfluidic device comprising a first polymer layer defining a first oxygen flow channel; a second polymer layer defining a first blood flow channel, the first blood flow channel further comprising a plurality of passive mixing elements spaced sequentially along a length of a surface of the first blood flow channel, wherein the plurality of passive mixing elements have a height or a depth less than about 30% of a height of the first blow flow channel; and a membrane separating the first oxygen flow channel and the first blood flow channel, the membrane allowing communication between the first oxygen flow channel and the first blood flow channel;
introducing partially deoxygenated blood into a proximal end of the microfluidic device;
flowing oxygen through the first oxygen flow channel;
flowing the partially deoxygenated blood through the first blood flow channel; and
receiving oxygenated blood at a distal end of the microfluidic device.
14. The method of claim 13 , further comprising:
collecting partially deoxygenated blood from a patient;
flowing the partially deoxygenated blood through the first blood flow channel to reoxygenate the blood; and
returning the reoxygenated blood to the patient.
15. The method of claim 14 , further comprising removing carbon dioxide from the partially deoxygenated blood as the partially deoxygenated blood flows through the first blood flow channel.
16. The method of claim 13 , further comprising flowing oxygen through the first oxygen flow channel from a first direction.
17. The method of claim 13 , further comprising flowing blood through the first blood flow channel in a second direction opposite to the first direction.
18. The method of claim 13 , further comprising flowing the blood through the first blood flow channel at 4-5 L/min.
19. The method of claim 13 , further comprising transferring oxygen into the blood at a rate of about 150-200 mL/min.