Stable lipid-comprising drug delivery complexes and methods for their production
View Patent ↗Novel stable, concentrated, biologically active and ready-to-use lipid-comprising drug delivery complexes and methods for their production are described. The biological activity of the complexes produced are comparable to the formulations prepared according to the prior art admixture method and upon purification, the complexes produced by the method of this invention are 50 to 500 fold more concentrated than the complexes formed by admixture. The method described herein provides for the large scale production of lipid-comprising drug delivery systems useful for gene therapy and other applications.
1. A noncovalent nucleic acid/lipid/polycation complex having a net positive charge at pH 6.0-8.0 comprising at least one cationic lipid and a polycation in a ratio of nucleic acid to lipid to polycation which results in the production of said complex, said polycation being selected from the group consisting of polyarginine, polyornithine, polybrene (hexadimethrine bromide), histone, cationic dendrimer, spermine, spermidine, protamine, and synthetic peptides derived from SV40 large T antigen and comprising a nuclear localization sequence,
wherein said polycation does not comprise a targeting factor directed to a particular tissue or cell type; and,
wherein the complex is formed by adding a nucleic acid to a mixture comprising the at least one cationic lipid and the polycation.
2. The complex of claim 1 , wherein the polycation has a molecular weight of 300 to 200,000 Daltons.
3. The complex of claim 1 , wherein the polycation is a histone.
4. The complex of claim 1 , wherein the polycation is a peptide derived from SV40 large T antigen.
5. The complex of claim 4 , wherein said peptide comprises a nuclear localization sequence.
6. The complex of claim 1 , wherein the polycation comprises spermine or spermidine.
7. The complex of claim 1 , wherein the polycation is a protamine.
8. The complex of claim 7 , wherein the protamine is selected from the group consisting of protamine free base, protamine phosphate, protamine chloride, protamine sulfate from salmon, and protamine sulfate from herring.
9. The complex of claim 1 , wherein the ratio of lipid to nucleic acid ranges from about 0.1 nmol to 200 nmol lipid per 1 μg nucleic acid.
10. The complex of claim 1 , wherein the ratio of lipid to nucleic acid ranges from about 1 nmol to about 25 nmol lipid per 1 μg nucleic acid.
11. The complex of claim 1 , wherein the polycation:nucleic acid ratio is about 0.01 μg to about 100 μg polycation to 1 μg nucleic acid.
12. A method for producing a noncovalent nucleic acid/lipid/polycation complex having a net positive charge at pH 6.0-8.0, the method comprising, mixing said nucleic acid with at least one cationic lipid and polycation in a ratio of nucleic acid to lipid to polycation which results in the production of said complex, said polycation being selected from the group consisting of polyarginine, polyornithine, polybrene (hexadimethrine bromide), histone, cationic dendrimer, spermine, spermidine, protamine, and synthetic peptides derived from a nuclear localization sequence of SV40 large T antigen,
wherein said polycation does not comprise a targeting factor directed to a particular tissue or cell type; and,
wherein said nucleic acid is added to a mixture of the at least one cationic lipid and the polycation.
13. The method of claim 12 , wherein the polycation has a molecular weight of 300 to 200,000 Daltons.
14. The method of claim 12 , wherein the polycation comprises a histone, spermine, spermidine, or a peptide derived from SV40 large T antigen.
15. The method of claim 12 , wherein the polycation is a protamine.
16. The method of claim 15 , wherein the protamine is selected from the group consisting of protamine free base, protamine phosphate, protamine chloride, protamine sulfate from salmon, and protamine sulfate from herring.
17. A method for delivering a nucleic acid to cells comprising contacting the cells with the noncovalent nucleic acid/lipid/polycation complex of claim 1 .