Method for extracting oil from sea cucumber material
A method for extracting oil from sea cucumber material. The sea cucumber material is mixed with water and the mixture is heated at a predetermined heating temperature for a predetermined heating time interval. The pH value and temperature of the mixture are adjusted to be compatible with an enzyme. A predetermined amount of the enzyme is then added to the mixture. The mixture is incubated at a predetermined incubation temperature for a predetermined incubation time interval. After the incubation the oil is removed from the mixture.
1. A method for extracting oil from sea cucumber waste material comprising:
mixing the sea cucumber waste material with water and heating the mixture at a predetermined heating temperature for a predetermined heating time interval;
adjusting pH value and temperature of the mixture to be compatible with an enzyme;
adding a predetermined amount of the enzyme to the mixture;
incubating the mixture at a predetermined incubation temperature for a predetermined incubation time interval; and
removing the oil from the mixture.
2. A method as defined in claim 1 comprising:
pasteurizing the mixture;
centrifuging the mixture producing an aqueous layer and an oil layer; and,
removing the oil layer after centrifuging.
3. A method as defined in claim 2 comprising adding a predetermined amount of a plant or animal based oil to the mixture before centrifuging.
4. A method as defined in claim 3 wherein the plant or animal based oil comprises safflower oil.
5. A method as defined in claim 2 comprising:
adding a predetermined amount of a plant or animal based oil to the aqueous phase;
centrifuging the mixture of the plant or animal based oil and the aqueous phase; and,
removing the oil layer after centrifuging.
6. A method as defined in claim 5 wherein the plant or animal based oil comprises safflower oil.
7. A method as defined in claim 1 comprising agitating the mixture during the incubation.
8. A method as defined in claim 2 comprising removing solid particles from the mixture before centrifuging the mixture.
9. A method as defined in claim 1 comprising purifying the removed oil.
10. A method as defined in claim 2 wherein the mixture is centrifuged while having a temperature proximate a pasteurizing temperature.
11. A method as defined in claim 1 wherein the sea cucumber waste material substantially comprises sea cucumber viscera.
12. A method as defined in claim 1 wherein the enzyme is one of a neutral and an alkaline protease.
13. A method as defined in claim 12 wherein the pH value is adjusted using sodium hydroxide (NaOH) or a phosphate buffer.
14. A method as defined in claim 1 comprising:
pasteurizing the mixture;
adding a first predetermined amount of a plant or animal based oil to the mixture;
centrifuging the mixture;
removing the oil layer after centrifuging;
adding a second predetermined amount of a plant or animal based oil to the remaining mixture;
centrifuging the remaining mixture; and,
removing the oil layer after centrifuging.
15. A compound comprising oil extracted from sea cucumber waste material using an enzyme.
16. A compound as defined in claim 15 comprising safflower oil.
17. A method for extracting oil from sea cucumber waste material comprising:
mixing the sea cucumber waste material with water and heating the mixture at a predetermined heating temperature for a predetermined heating time interval;
adjusting pH value and temperature of the mixture to be compatible with an enzyme;
adding a predetermined amount of the enzyme to the mixture;
incubating the mixture at a predetermined incubation temperature for a predetermined incubation time interval;
pasteurizing the mixture;
adding a predetermined amount of a plant or animal based oil to the mixture;
centrifuging the mixture; and,
removing the oil layer after centrifuging.
18. A method for extracting oil from sea cucumber waste material comprising:
mixing the sea cucumber waste material with water and heating the mixture at a predetermined heating temperature for a predetermined heating time interval;
adjusting pH value and temperature of the mixture to be compatible with an enzyme;
adding a predetermined amount of the enzyme to the mixture;
incubating the mixture at a predetermined incubation temperature for a predetermined incubation time interval;
pasteurizing the mixture;
adding a first predetermined amount of a plant or animal based oil to the mixture;
centrifuging the mixture;
removing the oil layer after centrifuging;
adding a second predetermined amount of a plant or animal based oil to the remaining mixture;
centrifuging the remaining mixture; and,
removing the oil layer after centrifuging.