HYALURONIC ACID-COLLAGEN MATRICES FOR TISSUE ENGINEERING
Hydrogels comprising a macromolecular matrix including a crosslinked combination of hyaluronic acid and collagen, are provided for tissue engineering applications.
1 . A hydrogel composition useful for treating a human or veterinary subject having a disorder characterized by tissue damage or loss, the composition comprising:
a hyaluronic acid component; and
a collagen component;
wherein the hyaluronic acid component is crosslinked to the collagen component by an ester bond or an amide bond.
2 . The composition of claim 1 having a weight ratio of the hyaluronic acid component to the collagen component of about 1 to about 7.
3 . The composition of claim 1 having a weight ratio of the hyaluronic acid component to the collagen component of about 2.
4 . The composition of claim 1 having a weight ratio of the hyaluronic acid component to the collagen component of about 3.
5 . The composition of claim 1 having a weight ratio of the hyaluronic acid component to the collagen component of about 4.
6 . The composition of claim 1 having a weight ratio of the hyaluronic acid component to the collagen component of about 6.
7 . The composition of claim 1 having a collagen concentration of at least about 3 mg/ml to about 12 mg/ml.
8 . The composition of claim 7 having a hyaluronic acid concentration of about 3 mg/ml to about 24 mg/ml.
9 . The composition of claim 1 having a collagen concentration of about 3 mg/mL.
10 . The composition of claim 9 having a hyaluronic acid concentration of about 3 mg/mL to about 20 mg/mL.
11 . The composition of claim 9 having a hyaluronic acid concentration of about 3 mg/mL.
12 . The composition of claim 9 having a hyaluronic acid concentration of about 12 mg/mL.
13 . The composition of claim 9 having a hyaluronic acid concentration of about 20 mg/mL.
14 . The composition of claim 1 having a collagen concentration of about 6 mg/mL.
15 . The composition of claim 14 having a hyaluronic acid concentration of about 12 mg/mL.
16 . The composition of claim 1 having a collagen concentration of about 8 mg/mL.
17 . The composition of claim 16 having a hyaluronic acid concentration of about 16 mg/mL.
18 . The composition of claim 1 having a collagen concentration of about 12 mg/mL.
19 . The composition of claim 18 having a hyaluronic acid concentration of about 12 mg/mL to about 24 mg/mL.
20 . The composition of claim 18 having a hyaluronic acid concentration of about 12 mg/mL.
21 . The composition of claim 18 having a hyaluronic acid concentration of about 24 mg/mL.
22 . A method for treating a human or veterinary subject having a disorder characterized by soft tissue damage or loss, the method comprising:
introducing into soft tissue in a patient, a hydrogel composition wherein the hydrogel composition comprises water and a crosslinked macromolecular matrix comprising
a hyaluronic acid component, and
a collagen component,
wherein the hyaluronic acid component is crosslinked to the collagen component by a crosslinking component, and
wherein the crosslinking component comprises a plurality of crosslink units, wherein at least a portion of the crosslink units comprise an ester bond or an amide bond.
23 . The method of claim 22 wherein the hydrogel composition has a weight ratio of the hyaluronic acid component to the collagen component of about 1 to about 7.
24 . The method of claim 22 wherein the hydrogel composition has a collagen concentration of about 3 mg/mL to about 12 mg/mL.
25 . The method of claim 24 wherein the hydrogel composition has a hyaluronic acid concentration of about 3 mg/mL to about 24 mg/mL.
26 . The method of claim 22 wherein the hydrogel composition has a collagen concentration of about 3 mg/mL and a hyaluronic acid concentration of about 3 mg/mL.
27 . The method of claim 22 wherein the hydrogel composition has a collagen concentration of about 3 mg/mL and a hyaluronic acid concentration of about 12 mg/mL.
28 . The method of claim 22 wherein the hydrogel composition has a collagen concentration of about 3 mg/mL and a hyaluronic acid concentration of about 16 mg/mL.
29 . The method of claim 22 wherein the hydrogel composition has a collagen concentration of about 3 mg/mL and a hyaluronic acid concentration of about 20 mg/mL.