Vmp-like sequences of pathogenic
The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic Borreliae, the use of the DNA sequences in recombinant vectors to express polypeptides, the encoded amino acid sequences, application of the DNA and amino acid sequences to the production of polypeptides as antigens for immunoprophylaxis, immunotherapy, and immunodiagnosis. Also disclosed are the use of the nucleic acid sequences as probes or primers for the detection of organisms causing Lyme disease, relapsing fever, or related disorders, and kits designed to facilitate methods of using the described polypeptides, DNA segments and antibodies.
1. An isolated antibody that binds immunologically to an epitope of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, or SEQ ID NO:30, said antibody being immobilized on a synthetic solid support.
2. The isolated antibody immobilized on a synthetic solid support of claim 1 , further defined as a monoclonal antibody.
3. The isolated antibody immobilized on a synthetic solid support of claim 1 , further defined as a polyclonal antibody.
4. The isolated antibody immobilized on a synthetic solid support of claim 1 , further defined as labeled with a label.
5. The isolated antibody immobilized on a synthetic solid support of claim 4 , wherein the antibody is further defined as conjugated to a secondary binding ligand.
6. The isolated antibody immobilized on a synthetic solid support of claim 4 , wherein the antibody is further defined as bound by a secondary antibody directed against the antibody.
7. The isolated antibody immobilized on a synthetic solid support of claim 1 , wherein the synthetic solid support is comprised in an affinity column.
8. The isolated antibody immobilized on a synthetic solid support of claim 1 , further defined as immunologically bound to a protein or polypeptide comprising an epitope of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, or SEQ ID NO:30.
9. The isolated antibody immobilized on a synthetic solid support of claim 4 , wherein the label is radioactive.
10. The isolated antibody immobilized on a synthetic solid support of claim 4 , wherein the label is chemiluminescent.
11. The isolated antibody immobilized on a synthetic solid support of claim 4 , wherein the label is colorimetric.
12. The isolated antibody immobilized on a synthetic solid support of claim 5 , wherein the secondary binding ligand is biotin.
13. The isolated antibody immobilized on a synthetic solid support of claim 5 , wherein the secondary binding ligand is avidin.
14. The isolated antibody immobilized on a synthetic solid support of claim 13 , wherein the secondary binding ligand is streptavidin.
15. An immunodetection kit comprising the isolated antibody immobilized on a synthetic solid support of claim 1 and a reagent for detecting antibody immunological binding.
16. The isolated antibody immobilized on a synthetic solid support of claim 1 , further defined as non-covalently immobilized.
17. The isolated antibody immobilized on a synthetic solid support of claim 1 , wherein the synthetic solid support is a well or plate.
18. The isolated antibody immobilized on a synthetic solid support of claim 1 , wherein the antibody is immobilized on the synthetic solid support by immunological binding to an antigen that is immobilized on the synthetic solid support.